Monocyte deactivation in neutropenic acute respiratory distress syndrome patients treated with granulocyte colony-stimulating factor.

Details

Serval ID
serval:BIB_CE3C7B882575
Type
Article: article from journal or magazin.
Collection
Publications
Title
Monocyte deactivation in neutropenic acute respiratory distress syndrome patients treated with granulocyte colony-stimulating factor.
Journal
Critical care
Author(s)
Mokart D., Kipnis E., Guerre-Berthelot P., Vey N., Capo C., Sannini A., Brun J.P., Blache J.L., Mege J.L., Blaise D., Guery B.P.
ISSN
1466-609X (Electronic)
ISSN-L
1364-8535
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
12
Number
1
Pages
R17
Language
english
Notes
Publication types: Clinical Trial ; Journal Article
Publication Status: ppublish
Abstract
In severely neutropenic septic acute respiratory distress syndrome (ARDS) patients, macrophages and monocytes are the last potentially remaining innate immune cells. We have previously shown, however, a deactivation of the alveolar macrophage in neutropenic septic ARDS patients. In the present study, we tried to characterize in vitro monocyte baseline cytokine production and responsiveness to lipopolysaccharide exposure.
Twenty-two consecutive patients with cancer were prospectively enrolled into a prospective observational study in an intensive care unit. All patients developed septic ARDS and were divided into two groups: neutropenic patients (n = 12) and non-neutropenic patients (n = 10). All of the neutropenic patients received granulocyte colony-stimulating factor whereas no patient in the non-neutropenic group received granulocyte colony-stimulating factor. We compared monocytes from neutropenic patients with septic ARDS with monocytes from non-neutropenic patients and healthy control individuals (n = 10). Peripheral blood monocytes were cultured, and cytokine levels (TNFalpha, IL-1beta, IL-6, IL-10, and IL-1 receptor antagonist) were assayed with and without lipopolysaccharide stimulation.
TNFalpha, IL-6, IL-10 and IL-1 receptor antagonist levels in unstimulated monocytes were lower in neutropenic patients compared with non-neutropenic patients. Values obtained in the healthy individuals were low as expected, comparable with neutropenic patients. In lipopolysaccharide-stimulated monocytes, both inflammatory and anti-inflammatory cytokine production were significantly lower in neutropenic patients compared with non-neutropenic patients and control individuals.
Consistent with previous results concerning alveolar macrophage deactivation, we observed a systemic deactivation of monocytes in septic neutropenic ARDS. This deactivation participates in the overall immunodeficiency and could be linked to sepsis, chemotherapy and/or the use of granulocyte colony-stimulating factor.
Keywords
Adult, Cytokines/biosynthesis, Female, Granulocyte Colony-Stimulating Factor/therapeutic use, Humans, Lipopolysaccharides, Macrophage Activation, Male, Middle Aged, Monocytes/metabolism, Neoplasms/complications, Neutropenia/complications, Neutropenia/drug therapy, Neutropenia/metabolism, Neutropenia/mortality, Respiratory Distress Syndrome/complications, Respiratory Distress Syndrome/drug therapy, Respiratory Distress Syndrome/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
29/04/2021 9:59
Last modification date
17/07/2023 13:39
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