Melanoma-expressed CD70 is involved in invasion and metastasis.

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Ressource 1Download: Pich et al. - 2016 - Melanoma Expressed-CD70 Is Regulated by RhoA and M.pdf (4701.60 [Ko])
State: Public
Version: Final published version
License: CC BY-NC-SA 4.0
Serval ID
serval:BIB_CE366D515440
Type
Article: article from journal or magazin.
Collection
Publications
Title
Melanoma-expressed CD70 is involved in invasion and metastasis.
Journal
British Journal of Cancer
Author(s)
Pich C., Sarrabayrouse G., Teiti I., Mariamé B., Rochaix P., Lamant L., Favre G., Maisongrosse V., Tilkin-Mariamé A.F.
ISSN
1532-1827 (Electronic)
ISSN-L
0007-0920
Publication state
Published
Issued date
12/2016
Volume
114
Number
1
Pages
63-70
Language
english
Notes
Publication types: Journal Article
Publication Status: published
Abstract
BACKGROUND: CD70 is a costimulatory molecule of the tumour necrosis factor family expressed in activated immune cells and some solid tumours. In lymphocytes CD70 triggers T cell-mediated cytotoxicity and mitogen-activated protein kinase phosphorylation.
METHODS: We evaluated the expression of CD70 in biopsies and melanoma cell lines. Using melanoma cell lines positive or not for CD70, we analysed CD70 function on melanoma progression.
RESULTS: We report CD70 expression in human melanoma cell lines and tumour cells from melanoma biopsies. This expression was observed in 95% of primary melanomas but only 37% of metastases. Both monomeric and trimeric forms of CD70 were detected in tumour cell membrane fractions, whereas cytoplasmic fractions contained almost exclusively monomeric CD70. In vitro and in vivo experiments demonstrated that CD70 expression inhibited melanoma cell migration, invasion and pulmonary metastasis implantation independently of the tumour immune microenvironment. Increasing the levels of the trimeric form of CD70 through monoclonal antibody binding led to an increase in CD70+ melanoma cell invasiveness through MAPK pathway activation, RhoE overexpression, ROCK1 and MYPT1 phosphorylation decrease, and stress fibres and focal adhesions disappearance.
CONCLUSIONS: Our results describe a new non-immunological function of melanoma-expressed CD70, which involves melanoma invasiveness through MAPK pathway, RhoE and cytoskeletal modulation.
Pubmed
Open Access
Yes
Create date
18/02/2016 12:12
Last modification date
03/03/2023 7:48
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