Population genetic analysis of Pseudomonas aeruginosa using multilocus sequence typing
Details
Serval ID
serval:BIB_CD4ED1045832
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Population genetic analysis of Pseudomonas aeruginosa using multilocus sequence typing
Journal
FEMS Immunology and Medical Microbiology
ISSN
0928-8244
Publication state
Published
Issued date
01/2005
Peer-reviewed
Oui
Volume
43
Number
1
Pages
29-35
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Abstract
To study the population genetic structure of Pseudomonas aeruginosa, we developed a multilocus sequence typing scheme. The sequences of internal fragments of seven housekeeping genes were obtained for 34 P. aeruginosa isolates from patients hospitalized in five different European cities. Twenty-six different allelic profiles were identified. The mean allelic diversity was 0.854 (range: 0.606-0.978), which was about six times greater than the results obtained with the multilocus enzyme electrophoresis method. Linkage disequilibrium was measured with the index of association. An index of 1.95+/-0.24 was calculated when all the strains were considered. This index was 1.76+/-0.27 when only one strain per sequence type was considered. Both results were different from 0, indicating linkage among loci, which means that the population structure of our set of P. aeruginosa isolates is clonal. The clonal structure of the population was also suggested by the congruence of the topology of the different trees obtained from the seven housekeeping genes. These results are in contrast to previous studies, finding a non clonal population structure. Since a small number of isolates was analyzed in this study, there might be a bias of selection which includes the possibility that they belong to widely disseminated epidemic clones. Another possibility is that recombination did not occurred homogeneously throughout the genome of P. aeruginosa, so that part of it has a clonal structure, while the remaining part of the genome is more frequently subject to recombination.
Keywords
Alleles
Bacterial Proteins/genetics
*Bacterial Typing Techniques
DNA, Bacterial
Epidemiology, Molecular
Genes, Bacterial
Humans
Molecular Sequence Data
Phylogeny
Pseudomonas Infections/microbiology
Pseudomonas aeruginosa/*classification/*genetics/isolation & purification
Sequence Analysis, DNA
Sequence Homology
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 15:20
Last modification date
20/08/2019 15:47