A rigorous behavioral testing platform for the assessment of radiation-induced neurological outcomes

Details

Serval ID
serval:BIB_CD4CAE330E1F
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
A rigorous behavioral testing platform for the assessment of radiation-induced neurological outcomes
Title of the book
Methods in Cell Biology
Author(s)
Drayson Olivia G.G., Vozenin Marie-Catherine, Limoli Charles L.
Publisher
Elsevier
ISBN
9780323990073
ISSN
0091-679X
ISSN-L
0091-679X
Publication state
Published
Issued date
2023
Peer-reviewed
Oui
Volume
180
Pages
177-197
Language
english
Abstract
Behavioral testing is a popular and reliable method of neurocognitive assessment of rodents but the lack of standard operating procedures has led to a high variation of protocols in use. Therefore, there exists a strong need to standardize protocols for a combined behavioral platform in order to maintain consistency across institutions and assist newcomers in the field. This paper provides details on the methodology of several behavioral tasks which have been validated in identifying radiation induced cognitive impairment as well as provide guidance on timescales and best practices. The cognitive assessments outlined here are optimized for rodent studies and either target learning and memory (open field task, object in updated location, novel object recognition, object in place, and temporal order) or mood and cognition (social interaction, elevated plus maze, light dark box, forced swim test, and fear extinction). We have utilized this platform successfully in evaluating cognitive injury induced by various radiation types, doses, fractionation schedules and also with ultra-high dose rate FLASH radiotherapy. Recommended materials and software are provided as well as advice on methods of data analysis. In this way a comprehensive behavioral platform is described with broad applicability to assess cognitive endpoints critical to therapeutic outcome.
Keywords
Animals, Fear/psychology, Behavior, Animal, Extinction, Psychological, Swimming, Behavior, Cognitive assessment, Memory, Mood, Radiation, Rodents
Pubmed
Web of science
Create date
06/11/2023 13:55
Last modification date
20/01/2024 7:11
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