Gene-expression analysis reveals that embryonic stem cells cultured under osteogenic conditions produce mineral non-specifically compared to marrow stromal cells or osteoblasts.

Details

Serval ID
serval:BIB_CD35FF441887
Type
Article: article from journal or magazin.
Collection
Publications
Title
Gene-expression analysis reveals that embryonic stem cells cultured under osteogenic conditions produce mineral non-specifically compared to marrow stromal cells or osteoblasts.
Journal
European cells & materials
Author(s)
Evans N.D., Swain R.J., Gentleman E., Gentleman M.M., Stevens M.M.
ISSN
1473-2262 (Electronic)
ISSN-L
1473-2262
Publication state
Published
Issued date
24/09/2012
Peer-reviewed
Oui
Volume
24
Pages
211-223
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Pluripotent cells, such as embryonic stem cells (ESCs), divide indefinitely and can differentiate to form mineralised nodules in response to osteogenic supplements. This suggests that they may be used as a cell source for bone replacement strategies. Here, we related the expression of osteogenic and chondrogenic genes in cultures of murine ESCs, marrow stromal cells (MSCs) and calvarial osteoblasts (OBs) cultured under osteogenic conditions to the biochemical composition and quantity of mineral formed. Mineralisation, measured by calcium sequestration, was >2-fold greater in ESC cultures than in either MSCs or OBs. Micro-Raman spectroscopy and spectral mapping revealed a lower mineral-to-matrix ratio and confirmed a more diffuse pattern of mineralisation in ESCs compared to MSCs and OBs. Baseline expression of chondrogenic and osteogenic genes was between 1 and 4 orders of magnitude greater in MSCs and OBs than in ESCs. Osteogenic culture of MSCs and OBs was accompanied by increases in osteogenic gene expression by factors of ~100 compared to only ~10 in ESCs. Consequentially, peak expression of osteogenic and chondrogenic genes was greater in MSCs and OBs than ESCs by factors of 100-1000, despite the fact that mineralisation was more extensive in ESCs than either MSCs or OBs. We also observed significant cell death in ESC nodules. We conclude that the mineralised material observed in cultures of murine ESCs during osteogenic differentiation may accumulate non-specifically, perhaps in necrotic cell layers, and that thorough characterisation of the tissue formed by ESCs must be achieved before these cells can be considered as a cell source for clinical applications.
Keywords
Animals, Calcification, Physiologic, Cells, Cultured, Chondrogenesis/genetics, Embryonic Stem Cells/metabolism, Female, Gene Expression Profiling, Mesenchymal Stem Cells/metabolism, Mice, Osteoblasts/metabolism, Osteogenesis/genetics
Pubmed
Web of science
Open Access
Yes
Create date
12/01/2024 10:14
Last modification date
13/01/2024 7:10
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