Compact solid-state CMOS single-photon detector array for in vivo NIR fluorescence lifetime oncology measurements.

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Serval ID
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Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Compact solid-state CMOS single-photon detector array for in vivo NIR fluorescence lifetime oncology measurements.
Journal
Biomedical Optics Express
Author(s)
Homulle H.A., Powolny F., Stegehuis P.L., Dijkstra J., Li D.U., Homicsko K., Rimoldi D., Muehlethaler K., Prior J.O., Sinisi R., Dubikovskaya E., Charbon E., Bruschini C.
ISSN
2156-7085 (Electronic)
ISSN-L
2156-7085
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
7
Number
5
Pages
1797-1814
Language
english
Abstract
In near infrared fluorescence-guided surgical oncology, it is challenging to distinguish healthy from cancerous tissue. One promising research avenue consists in the analysis of the exogenous fluorophores' lifetime, which are however in the (sub-)nanosecond range. We have integrated a single-photon pixel array, based on standard CMOS SPADs (single-photon avalanche diodes), in a compact, time-gated measurement system, named FluoCam. In vivo measurements were carried out with indocyanine green (ICG)-modified derivatives targeting the αvβ 3 integrin, initially on a genetically engineered mouse model of melanoma injected with ICG conjugated with tetrameric cyclic pentapeptide (ICG-E[c(RGD f K)4]), then on mice carrying tumour xenografts of U87-MG (a human primary glioblastoma cell line) injected with monomeric ICG-c(RGD f K). Measurements on tumor, muscle and tail locations allowed us to demonstrate the feasibility of in vivo lifetime measurements with the FluoCam, to determine the characteristic lifetimes (around 500 ps) and subtle lifetime differences between bound and unbound ICG-modified fluorophores (10% level), as well as to estimate the available photon fluxes under realistic conditions.
Pubmed
Web of science
Create date
03/06/2016 18:32
Last modification date
20/08/2019 16:47
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