Purification and in vitro transcription of a transfer RNA gene

Details

Serval ID
serval:BIB_CCFCF2909048
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Purification and in vitro transcription of a transfer RNA gene
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Daniel  V., Beckmann  J. S., Sarid  S., Grimberg  J. I., Herzberg  M., Littauer  U. Z.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
09/1971
Volume
68
Number
9
Pages
2268-72
Notes
Journal Article --- Old month value: Sep
Abstract
A gene specifying tyrosine transfer RNA has been purified and transcribed in vitro. The purification procedure made use of two specialized transducing phages carrying the tRNA(Tyr) gene of Escherichia coli inserted into their DNA in opposite orientations. The separated heavy strands of the two phages were annealed and the single-stranded tails of the resulting hybrid were removed by digestion with Neurospora endonuclease. The size of the purified double-stranded structures was determined by electron microscopy. These isolated duplexes served as template for the in vitro transcription of tRNA(Tyr)-like molecules.
Keywords
Centrifugation, Density Gradient Coliphages DNA, Bacterial/*isolation & purification Deoxyribonucleases Escherichia coli *Genes *Genetic Code Microscopy, Electron Molecular Weight Nucleic Acid Hybridization *RNA, Transfer/biosynthesis Transduction, Genetic Tyrosine
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 17:18
Last modification date
20/08/2019 16:47
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