Identification and characterization of additional members of the cytochrome P450 multigene family CYP52 of Candida tropicalis

Details

Serval ID
serval:BIB_CCE17FF9894A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification and characterization of additional members of the cytochrome P450 multigene family CYP52 of Candida tropicalis
Journal
DNA and Cell Biology
Author(s)
Seghezzi  W., Meili  C., Ruffiner  R., Kuenzi  R., Sanglard  D., Fiechter  A.
ISSN
1044-5498 (Print)
Publication state
Published
Issued date
12/1992
Volume
11
Number
10
Pages
767-80
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
Using different DNA probes from the first two previously described alkane-inducible cytochrome P450 genes of the Candida tropicalis CYP52 gene family, we isolated five independent additional members by screening a genomic library under low-stringency conditions. These genes are not allelic variants and, when taken gogether, constitute the largest gene family known in this organism. The seven members of this gene family are located on four different chromosomes and four of them are tandemly arranged on the C. tropicalis genome. The products of the seven genes, alk1 to alk7, were compared to each other and revealed a high degree of divergence: the two most diverged proteins exhibit a sequence identity of only 32%. Six of the seven genes were shown to be induced by a variety of different aliphatic carbon sources but repressed when the organism was grown on glucose. Three of the five additional CYP52 genes could be successfully expressed in Saccharomyces cerevisiae and display different substrate specificities in in vitro assays with model substrates: alk2 and alk3 exhibit a strong preference for hexadecane, while alk4 and alk5 preferentially hydroxylate lauric acid.
Keywords
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Candida/*enzymology Carbon/metabolism Chromosome Mapping Cloning, Molecular Cytochrome P-450 Enzyme System/*genetics/metabolism DNA, Fungal Electrophoresis, Polyacrylamide Gel Fungal Proteins Gene Expression Genes, Fungal Hydroxylation Molecular Sequence Data *Multigene Family Restriction Mapping Saccharomyces cerevisiae/genetics Spectrum Analysis
Pubmed
Web of science
Create date
25/01/2008 14:40
Last modification date
20/08/2019 15:47
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