P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons.

Details

Serval ID
serval:BIB_CCB23991CFF5
Type
Article: article from journal or magazin.
Collection
Publications
Title
P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons.
Journal
The Journal of comparative neurology
Author(s)
Florenzano F., Viscomi M.T., Mercaldo V., Longone P., Bernardi G., Bagni C., Molinari M., Carrive P.
ISSN
0021-9967 (Print)
ISSN-L
0021-9967
Publication state
Published
Issued date
01/09/2006
Volume
498
Number
1
Pages
58-67
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Neurophysiologic data suggest that orexin neurons are directly excited by ATP through purinergic receptors (P2XR). Anatomical studies, though reporting P2XR in the hypothalamus, did not describe it in the perifornical hypothalamic area, where orexinergic neurons are located. Here we report the presence of the P2X(2)R subunit in the rat perifornical hypothalamus and demonstrate that hypothalamic orexin neurons express the P2X(2)R. Double immunohistochemistry showed that virtually all orexin-immunoreactive neurons are also P2X(2)R immunoreactive, whereas 80% of P2X(2)R-immunoreactive neurons are also orexin positive. Triple-labeling experiments, combining fluorescence in situ hybridization for P2X(2)R mRNA and P2X(2)R/orexin double immunofluorescence, confirmed these findings. In addition, in situ hybridization demonstrated that P2X(2)R mRNA is localized in cellular processes of orexinergic neurons. The present data support neurophysiologic findings on ATP modulation of orexinergic function and provide direct evidence that the entire population of orexin neurons expresses a P2XR subtype, namely, P2X(2)R. Thus, purinergic transmission might intervene in modulating key functions known to be controlled by the orexinergic system, such as feeding behavior and arousal.

Keywords
Adenosine Triphosphate/metabolism, Animals, Arousal/physiology, Dendrites/metabolism, Dendrites/ultrastructure, Feeding Behavior/physiology, Fluorescent Antibody Technique/methods, Hypothalamus/cytology, Hypothalamus/metabolism, Immunohistochemistry, In Situ Hybridization, Intracellular Signaling Peptides and Proteins/metabolism, Male, Neural Pathways/cytology, Neural Pathways/metabolism, Neurons/metabolism, Neuropeptides/metabolism, Orexin Receptors, Orexins, RNA, Messenger/metabolism, Rats, Rats, Wistar, Receptors, G-Protein-Coupled, Receptors, Neuropeptide, Receptors, Purinergic P2/genetics, Receptors, Purinergic P2/metabolism, Receptors, Purinergic P2X2
Pubmed
Create date
06/03/2017 18:23
Last modification date
20/08/2019 16:47
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