Differential expression of the cationic amino acid transporter 2(B) in the adult rat brain

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serval:BIB_CC9D831C9A1F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential expression of the cationic amino acid transporter 2(B) in the adult rat brain
Journal
Brain Research. Molecular Brain Research
Author(s)
Braissant O., Gotoh T., Loup M., Mori M., Bachmann C.
ISSN
0169-328X (Print)
Publication state
Published
Issued date
2001
Volume
91
Number
1-2
Pages
189-195
Notes
Journal Article
Abstract
L-Arginine is a substrate for the synthesis of proteins, nitric oxide (NO), creatine, urea, proline, glutamate, polyamines and agmatine. In the central nervous system (CNS), arginine is extracted from the blood and exchanged by cells through carriers called cationic amino acid transporters (CAT) and belonging to the so-called system y+. In order to better understand the arginine transport in the CNS, we studied in detail the regional distribution of the cells expressing the CAT2(B) transcript in the adult rat brain by non-radioisotopic in situ hybridization. We show that CAT2(B) is expressed in neurons and oligodendrocytes throughout the brain, but is not detected in astrocytes. The pattern of localization of CAT2(B) in the normal adult rat brain fits closely that of CRT1, a specific creatine transporter. Our study demonstrates that the in vivo expression of CAT2(B) differs from that reported in vitro, implying that local cellular interactions should be taken into account in studies of gene regulation of the CAT2(B) gene. Our work suggests that CAT2(B) may play a role in case of increased NO production as well as arginine or creatine deficiency in the brain.
Keywords
Age Factors Amino Acid Transport Systems, Basic Animals Arginine/metabolism Brain Chemistry/*physiology Carrier Proteins/analysis/*genetics/metabolism Creatine/metabolism Gene Expression/physiology Immunohistochemistry In Situ Hybridization Lipopolysaccharides/pharmacology Membrane Proteins/analysis/*genetics/metabolism Neurons/chemistry/metabolism Nitric Oxide/metabolism Oligodendroglia/chemistry/metabolism RNA, Messenger/analysis Rats
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:21
Last modification date
20/08/2019 16:47
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