CD36 is important for adipocyte recruitment and affects lipolysis.

Details

Serval ID
serval:BIB_CC571E366BD3
Type
Article: article from journal or magazin.
Collection
Publications
Title
CD36 is important for adipocyte recruitment and affects lipolysis.
Journal
Obesity
Author(s)
Vroegrijk I.O., van Klinken J.B., van Diepen J.A., van den Berg S.A., Febbraio M., Steinbusch L.K., Glatz J.F., Havekes L.M., Voshol P.J., Rensen P.C., van Dijk K.W., van Harmelen V.
ISSN
1930-739X (Electronic)
ISSN-L
1930-7381
Publication state
Published
Issued date
10/2013
Peer-reviewed
Oui
Volume
21
Number
10
Pages
2037-2045
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The scavenger receptor CD36 facilitates the cellular uptake of long-chain fatty acids. As CD36-deficiency attenuates the development of high fat diet (HFD)-induced obesity, the role of CD36-deficiency in preadipocyte recruitment and adipocyte function was set out to characterize.
Fat cell size and number were determined in gonadal, visceral, and subcutaneous adipose tissue of CD36(-/-) and WT mice after 6 weeks on HFD. Basal lipolysis and insulin-inhibited lipolysis were investigated in gonadal adipose tissue.
CD36(-/-) mice showed a reduction in adipocyte size in all fat pads. Gonadal adipose tissue also showed a lower total number of adipocytes because of a lower number of very small adipocytes (diameter <50 μm). This was accompanied by an increased pool of preadipocytes, which suggests that CD36-deficiency reduces the capacity of preadipocytes to become adipocytes. Regarding lipolysis, in adipose tissue from CD36(-/-) mice, cAMP levels were increased and both basal and 8-bromo-cAMP stimulated lipolysis were higher. However, insulin-mediated inhibition of lipolysis was more potent in CD36(-/-) mice.
These results indicate that during fat depot expansion, CD36-deficiency negatively affects preadipocyte recruitment and that in mature adipocytes, CD36-deficiency is associated with increased basal lipolysis and insulin responsiveness.
Keywords
8-Bromo Cyclic Adenosine Monophosphate/metabolism, Adipocytes/physiology, Adipose Tissue/metabolism, Animals, CD36 Antigens/deficiency, CD36 Antigens/physiology, Cell Differentiation, Cell Size, Diet, High-Fat/adverse effects, Fatty Acids/metabolism, Insulin/metabolism, Lipolysis/physiology, Liver/cytology, Liver/metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Obesity/metabolism, Triglycerides/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
18/07/2013 11:21
Last modification date
26/02/2024 17:19
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