Dual requirement for the EcR/USP nuclear receptor and the dGATAb factor in an ecdysone response in Drosophila melanogaster.

Details

Serval ID
serval:BIB_CC41F8F136BE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dual requirement for the EcR/USP nuclear receptor and the dGATAb factor in an ecdysone response in Drosophila melanogaster.
Journal
Molecular and cellular biology
Author(s)
Brodu V., Mugat B., Roignant J.Y., Lepesant J.A., Antoniewski C.
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Publication state
Published
Issued date
08/1999
Peer-reviewed
Oui
Volume
19
Number
8
Pages
5732-5742
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The EcR/USP nuclear receptor controls Drosophila metamorphosis by activating complex cascades of gene transcription in response to pulses of the steroid hormone ecdysone at the end of larval development. Ecdysone release provides a ubiquitous signal for the activation of the receptor, but a number of its target genes are induced in a tissue- and stage-specific manner. Little is known about the molecular mechanisms involved in this developmental modulation of the EcR/USP-mediated pathway. Fbp1 is a good model of primary ecdysone response gene expressed in the fat body for addressing this question. We show here that the dGATAb factor binds to three target sites flanking an EcR/USP binding site in a 70-bp enhancer that controls the tissue and stage specificity of Fbp1 transcription. We demonstrate that one of these sites and proper expression of dGATAb are required for specific activation of the enhancer in the fat body. In addition, we provide further evidence that EcR/USP plays an essential role as a hormonal timer. Our study provides a striking example of the integration of molecular pathways at the level of a tissue-specific hormone response unit.
Keywords
Animals, Base Sequence, Binding Sites, DNA-Binding Proteins/physiology, Drosophila Proteins, Drosophila melanogaster/genetics, Drosophila melanogaster/growth & development, Ecdysone/pharmacology, Enhancer Elements, Genetic, Fat Body/metabolism, Fungal Proteins/physiology, GATA Transcription Factors, Gene Expression Regulation, Developmental/physiology, Insect Proteins/biosynthesis, Insect Proteins/genetics, Larva, Macromolecular Substances, Molecular Sequence Data, Organ Specificity, Protein Binding, Saccharomyces cerevisiae Proteins, Time Factors, Transcription Factors/physiology
Pubmed
Web of science
Open Access
Yes
Create date
28/10/2019 13:04
Last modification date
29/10/2019 6:26
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