5'AMP-activated protein kinase alpha deficiency enhances stress-induced apoptosis in BHK and PC12 cells

Details

Serval ID
serval:BIB_CBB2EB681B2D
Type
Article: article from journal or magazin.
Collection
Publications
Title
5'AMP-activated protein kinase alpha deficiency enhances stress-induced apoptosis in BHK and PC12 cells
Journal
Journal of Cellular and Molecular Medicine
Author(s)
Shaw  M. M., Gurr  W. K., McCrimmon  R. J., Schorderet  D. F., Sherwin  R. S.
ISSN
1582-1838 (Print)
Publication state
Published
Issued date
04/2007
Volume
11
Number
2
Pages
286-98
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't --- Old month value: Mar-Apr
Abstract
5'AMP-activated protein kinase (AMPK) activation occurs under a variety of stress conditions but the role of this enzyme in the promotion or inhibition of stress-induced cell death is unclear. To address this issue, we transformed two different cell lines with shRNA-expressing plasmids, targeting the alpha subunit of AMPK, and verified AMPKalpha downregulation. The cell lines were then stressed by exposure to medium without glucose (PC12 cells) or with the viral thymidine kinase-specific DNA replication inhibitors: acyclovir, penciclovir and ganciclovir (herpes simplex virus thymidine kinase-expressing Baby Hamster Kidney cells). In non-AMPK-downregulated cells, these stress treatments induced AMPK upregulation and phosphorylation, leaving open the question whether the association of AMPK activation with stress-induced cell death reflects a successful death-promoting or an ineffective death-inhibiting activity. In AMPKalpha-deficient cells (expressing AMPKalpha-specific shRNAs or treated with Compound C) exposure to low glucose medium or DNA replication inhibitors led to an enhancement of cell death, indicating that, under the conditions examined, the role of activated AMPK is not to promote, but to protect from or delay stress-induced cell death.
Keywords
Animals Apoptosis/*physiology Cell Line Cell Line, Transformed Cell Transformation, Viral Cricetinae Enzyme Activation Multienzyme Complexes/*deficiency/genetics *Oxidative Stress PC12 Cells Protein-Serine-Threonine Kinases/*deficiency/genetics Rats
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 13:59
Last modification date
20/08/2019 16:46
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