OmpA family proteins and Pmp-like autotransporter: new adhesins of Waddlia chondrophila.

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Serval ID
serval:BIB_CBA1FA9E394E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
OmpA family proteins and Pmp-like autotransporter: new adhesins of Waddlia chondrophila.
Journal
Pathogens and Disease
Author(s)
Kebbi-Beghdadi C., Domröse A., Becker E., Cisse O.H., Hegemann J.H., Greub G.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
73
Number
6
Pages
ftv035
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Waddlia chondrophila is a obligate intracellular bacterium belonging to the Chlamydiales order, a clade that also includes the well-known classical Chlamydia responsible for a number of severe human and animal diseases. Waddlia is an emerging pathogen associated with adverse pregnancy outcomes in humans and abortion in ruminants. Adhesion to the host cell is an essential prerequisite for survival of every strict intracellular bacteria and, in classical Chlamydia, this step is partially mediated by polymorphic outer membrane proteins (Pmps), a family of highly diverse autotransporters that represent about 15% of the bacterial coding capacity. Waddlia chondrophila genome however only encodes one putative Pmp-like protein. Using a proteomic approach, we identified several bacterial proteins potentially implicated in the adhesion process and we characterized their expression during the replication cycle of the bacteria. In addition, we demonstrated that the Waddlia Pmp-like autotransporter as well as OmpA2 and OmpA3, two members of the extended Waddlia OmpA protein family, exhibit adhesive properties on epithelial cells. We hypothesize that the large diversity of the OmpA protein family is linked to the wide host range of these bacteria that are able to enter and multiply in various host cells ranging from protozoa to mammalian and fish cells.
Keywords
Adhesins, Bacterial/genetics, Adhesins, Bacterial/metabolism, Animals, Bacterial Adhesion, Bacterial Outer Membrane Proteins/genetics, Bacterial Outer Membrane Proteins/metabolism, Cell Line, Chlamydiales/genetics, Chlamydiales/metabolism, Epithelial Cells/microbiology, Humans, Type V Secretion Systems/genetics, Type V Secretion Systems/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
03/11/2015 18:46
Last modification date
25/09/2019 6:10
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