Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts.

Details

Serval ID
serval:BIB_CB540C67D22D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts.
Journal
Science
Author(s)
Corthésy B., Hipskind R., Theulaz I., Wahli W.
ISSN
0036-8075[print], 0036-8075[linking]
Publication state
Published
Issued date
03/1988
Volume
239
Number
4844
Pages
1137-1139
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
One approach to analyzing the molecular mechanisms of gene expression in vivo is to reconstitute these events in cell-free systems in vitro. Although there is some evidence for tissue-specific transcription in vitro, transcriptionally active extracts that mimic a steroid hormone-dependent enhancement of transcription have not been described. In the study reported here, nuclear extracts of liver from the frog Xenopus laevis were capable of estrogen-dependent induction of a homologous vitellogenin promoter that contained the estrogen-responsive element.
Keywords
Acetyltransferases/genetics, Animals, Cell Nucleus/metabolism, Chloramphenicol O-Acetyltransferase, DNA, Recombinant, Estradiol/pharmacology, Female, Hela Cells/metabolism, Humans, Liver/ultrastructure, Male, Promoter Regions, Genetic, RNA Polymerase II/metabolism, Simian virus 40/genetics, Templates, Genetic, Transcription, Genetic/drug effects, Vitellogenins/genetics, Xenopus laevis
Pubmed
Web of science
Create date
25/01/2008 15:53
Last modification date
20/08/2019 16:46
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