Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16.
Details
Serval ID
serval:BIB_CB46CEDE7D28
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16.
Journal
Journal of Virology
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Publication state
Published
Issued date
1991
Volume
65
Number
12
Pages
7078-7083
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
To examine the biological properties of the bovine papillomavirus type 1 (BPV) and human papillomavirus type 16 (HPV16) E5 genes, each was cloned separately into a retroviral expression vector and helper-free recombinant viruses were generated in packaging cell lines. The BPV E5 retroviruses efficiently caused morphologic and tumorigenic transformation of cultured lines of murine fibroblasts, whereas the HPV16 E5 viruses were inactive in these assays. In contrast, infection of the p117 established line of murine epidermal keratinocytes with either the BPV or the HPV16 E5 retrovirus resulted in the generation of tumorigenic cells. Pam212 murine keratinocytes were also transformed to tumorigenicity by the HPV16 E5 gene but not by the gene carrying a frameshift mutation. These results establish that the HPV16 E5 gene is a transforming gene in cells related to its normal host epithelial cells.
Keywords
3T3 Cells, Animals, Bovine papillomavirus 1/genetics, Bovine papillomavirus 1/isolation & purification, Cattle, Cell Line, Cell Transformation, Neoplastic, Gene Expression, Genes, Viral, Genetic Vectors, Humans, Keratinocytes/cytology, Keratinocytes/pathology, Mice, Mice, Nude, Neoplasm Transplantation, Papillomaviridae/genetics, Papillomaviridae/isolation & purification, Tumor Virus Infections/microbiology, Tumor Virus Infections/pathology
Pubmed
Web of science
Create date
24/01/2008 15:58
Last modification date
20/08/2019 16:46