First Insights into Human Fingertip Regeneration by Echo-Doppler Imaging and Wound Microenvironment Assessment.

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State: Public
Version: Final published version
Serval ID
serval:BIB_CB4046B499D3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
First Insights into Human Fingertip Regeneration by Echo-Doppler Imaging and Wound Microenvironment Assessment.
Journal
International journal of molecular sciences
Author(s)
Jafari P., Muller C., Grognuz A., Applegate L.A., Raffoul W., di Summa P.G., Durand S.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
13/05/2017
Peer-reviewed
Oui
Volume
18
Number
5
Pages
0
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Fingertip response to trauma represents a fascinating example of tissue regeneration. Regeneration derives from proliferative mesenchymal cells (blastema) that subsequently differentiate into soft and skeletal tissues. Clinically, conservative treatment of the amputated fingertip under occlusive dressing can shift the response to tissue loss from a wound repair process towards regeneration. When analyzing by Immunoassay the wound exudate from occlusive dressings, the concentrations of brain-derived neurotrophic factor (BDNF) and leukemia inhibitory factor (LIF) were higher in fingertip exudates than in burn wounds (used as controls for wound repair versus regeneration). Vascular endothelial growth factor A (VEGF-A) and platelet-derived growth factor (PDGF) were highly expressed in both samples in comparable levels. In our study, pro-inflammatory cytokines were relatively higher expressed in regenerative fingertips than in the burn wound exudates while chemokines were present in lower levels. Functional, vascular and mechanical properties of the regenerated fingertips were analyzed three months after trauma and the data were compared to the corresponding fingertip on the collateral uninjured side. While sensory recovery and morphology (pulp thickness and texture) were similar to uninjured sides, mechanical parameters (elasticity, vascularization) were increased in the regenerated fingertips. Further studies should be done to clarify the importance of inflammatory cells, immunity and growth factors in determining the outcome of the regenerative process and its influence on the clinical outcome.

Keywords
Adult, Aged, Brain-Derived Neurotrophic Factor/genetics, Burns/genetics, Burns/physiopathology, Cell Differentiation/genetics, Exudates and Transudates/immunology, Exudates and Transudates/metabolism, Exudates and Transudates/physiology, Finger Injuries/genetics, Finger Injuries/physiopathology, Humans, Leukemia Inhibitory Factor/genetics, Male, Mesenchymal Stromal Cells/metabolism, Mesenchymal Stromal Cells/physiology, Middle Aged, Occlusive Dressings, Platelet-Derived Growth Factor/genetics, Regeneration/genetics, Vascular Endothelial Growth Factor A/genetics, Wound Healing/genetics, Wound Healing/physiology, Doppler imaging, angiogenesis, clinical assessment, fingertip regeneration
Pubmed
Web of science
Open Access
Yes
Create date
23/05/2017 16:46
Last modification date
20/08/2019 15:46
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