Testing human hair for cannabis. II. Identification of THC-COOH by GC-MS-NCI as a unique proof.

Details

Serval ID
serval:BIB_CB3FF5FD42A0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Testing human hair for cannabis. II. Identification of THC-COOH by GC-MS-NCI as a unique proof.
Journal
Journal of Forensic Sciences
Author(s)
Kintz P., Cirimele V., Mangin P.
ISSN
0022-1198[print], 0022-1198[linking]
Publication state
Published
Issued date
1995
Volume
40
Number
4
Pages
619-622
Language
english
Abstract
To validate information on cannabis use, 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic (THC-COOH) was investigated in human hair. The identification of THC-COOH in hair would document cannabis use more effectively than the detection of the parent drug which might have come from environmental exposure in a smoky atmosphere. Samples (100 mg) were decontaminated with methylene chloride and destroyed by incubation in 1 mL of 1 N sodium hydroxide for 30 min at 95 degrees C in presence of 10 ng of THC-COOH-d3. After cooling, samples were extracted by n-hexane/ethyl acetate after acidification with acetic acid. After derivatization by PFPA-PFP-OH of the dry extract, drugs were separated on a HP1 capillary column, and detected by mass spectrometry (m/z 602) using negative chemical ionization with methane as reagent gas. Among 30 samples obtained from subjects deceased from fatal heroin overdose, 17 tested positive for THC-COOH, in the range 0.02-0.39 ng/mg, with an average of 0.12 ng/mg.
Keywords
Adult, Gas Chromatography-Mass Spectrometry/methods, Hair/chemistry, Humans, Marijuana Abuse, Tetrahydrocannabinol/analogs & derivatives, Tetrahydrocannabinol/analysis
Pubmed
Create date
19/10/2010 15:39
Last modification date
20/08/2019 15:46
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