Exovesicles from human activated dendritic cells fuse with resting dendritic cells, allowing them to present alloantigens.

Details

Serval ID
serval:BIB_CB1F07D41564
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Exovesicles from human activated dendritic cells fuse with resting dendritic cells, allowing them to present alloantigens.
Journal
American Journal of Pathology
Author(s)
Obregon C., Rothen-Rutishauser B., Gitahi S.K., Gehr P., Nicod L.P.
ISSN
0002-9440
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
169
Number
6
Pages
2127-2136
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Dendritic cells (DCs) can release microvesicles, but the latter's numbers, size, and fate are unclear. Fluorescently labeled DCs were visualized by laser-scanning microscopy. Using a Surpass algorithm, we were able to identify and quantify per cell several hundred microvesicles released from the surface of stimulated DCs. We show that most of these microvesicles are not of endocytic origin but result from budding of the plasma membrane, hence their name, exovesicle. Using a double vital staining, we show that exovesicles isolated from activated DCs can fuse with the membrane of resting DCs, thereby allowing them to present alloantigens to lymphocytes. We concluded that, within a few hours from their release, exovesicles may amplify local or distant adaptive immunological response.
Keywords
Antigen Presentation, Cell Differentiation, Cell Membrane/physiology, Coculture Techniques, Cytoplasmic Vesicles/immunology, Cytoplasmic Vesicles/physiology, Dendritic Cells/immunology, Dendritic Cells/ultrastructure, Humans, Isoantigens/physiology, Lipopolysaccharides/pharmacology, Microscopy, Confocal/methods, Monocytes/physiology
Pubmed
Web of science
Create date
12/02/2010 15:47
Last modification date
20/08/2019 15:45
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