Human MAIT cells exit peripheral tissues and recirculate via lymph in steady state conditions.
Details
Serval ID
serval:BIB_CAF34EE5BB0E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Human MAIT cells exit peripheral tissues and recirculate via lymph in steady state conditions.
Journal
JCI insight
ISSN
2379-3708 (Electronic)
ISSN-L
2379-3708
Publication state
Published
Issued date
05/04/2018
Peer-reviewed
Oui
Volume
3
Number
7
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Abstract
Mucosal-associated invariant T cells (MAIT cells) recognize bacterial metabolites as antigen and are found in blood and tissues, where they are poised to contribute to barrier immunity. Recent data demonstrate that MAIT cells located in mucosal barrier tissues are functionally distinct from their blood counterparts, but the relationship and circulation of MAIT cells between blood and different tissue compartments remains poorly understood. Previous studies raised the possibility that MAIT cells do not leave tissue and may either be retained or undergo apoptosis. To directly address if human MAIT cells exit tissues, we collected human donor-matched thoracic duct lymph and blood and analyzed MAIT cell phenotype, transcriptome, and T cell receptor (TCR) diversity by flow cytometry and RNA sequencing. We found that MAIT cells were present in the lymph, despite being largely CCR7- in the blood, thus indicating that MAIT cells in the lymph migrated from tissues and were capable of exiting tissues to recirculate. Importantly, MAIT cells in the lymph and blood had highly overlapping clonotype usage but distinct transcriptome signatures, indicative of differential activation states.
Keywords
Adolescent, Adult, Aged, Cell Separation, Child, Child, Preschool, Flow Cytometry, Gene Expression Profiling, Humans, Immunity, Mucosal, Lymph/cytology, Lymph/immunology, Middle Aged, Mucosal-Associated Invariant T Cells/immunology, Mucosal-Associated Invariant T Cells/metabolism, Mucous Membrane/cytology, Mucous Membrane/immunology, Receptors, Antigen, T-Cell/metabolism, Thoracic Duct, Young Adult, Cell migration/adhesion, Cellular immune response, Immunology, Lymph
Pubmed
Web of science
Open Access
Yes
Create date
28/02/2022 12:45
Last modification date
23/03/2024 8:24