Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells.

Details

Serval ID
serval:BIB_CAD99D5D3B4F
Type
Article: article from journal or magazin.
Collection
Publications
Title
Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells.
Journal
The Journal of cell biology
Author(s)
Junyent S., Reeves J., Gentleman E., Habib S.J.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
05/04/2021
Peer-reviewed
Oui
Volume
220
Number
4
Pages
e202005095
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
To coordinate cell fate with changes in spatial organization, stem cells (SCs) require specific and adaptable systems of signal exchange and cell-to-cell communication. Pluripotent embryonic stem cells (ESCs) use cytonemes to pair with trophoblast stem cells (TSCs) and form synthetic embryonic structures in a Wnt-dependent manner. How these interactions vary with pluripotency states remains elusive. Here we show that ESC transition to an early primed ESC (pESC) state reduces their pairing with TSCs and impairs synthetic embryogenesis. pESCs can activate the Wnt/β-catenin pathway in response to soluble Wnt ligands, but their cytonemes form unspecific and unstable interactions with localized Wnt sources. This is due to an impaired crosstalk between Wnt and glutamate receptor activity and reduced generation of Ca2+ transients on the cytonemes upon Wnt source contact. Induced iGluR activation can partially restore cytoneme function in pESCs, while transient overexpression of E-cadherin improves pESC-TSC pairing. Our results illustrate how changes in pluripotency state alter the mechanisms SCs use to self-organize.
Keywords
Animals, Cell Communication, Cell Line, Embryonic Development, Mice, Mouse Embryonic Stem Cells/cytology, Mouse Embryonic Stem Cells/metabolism, Trophoblasts/cytology, Trophoblasts/metabolism, Wnt Signaling Pathway
Pubmed
Web of science
Open Access
Yes
Create date
12/01/2024 11:14
Last modification date
13/01/2024 8:10
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