Identification of a conserved region of Plasmodium falciparum MSP3 targeted by biologically active antibodies to improve vaccine design

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Serval ID
serval:BIB_CAC01C95C9AF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of a conserved region of Plasmodium falciparum MSP3 targeted by biologically active antibodies to improve vaccine design
Journal
Journal of Infectious Diseases
Author(s)
Singh  S., Soe  S., Mejia  J. P., Roussilhon  C., Theisen  M., Corradin  G., Druilhe  P.
ISSN
0022-1899 (Print)
Publication state
Published
Issued date
09/2004
Volume
190
Number
5
Pages
1010-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 1
Abstract
Merozoite surface protein 3 (MSP3) is a target of antibody-dependent cellular inhibition (ADCI), a protective mechanism against Plasmodium falciparum malaria. From the C-terminal half of the molecule, 6 overlapping peptides were chosen to characterize human immune responses. Each peptide defined at least 1 non-cross-reactive B cell epitope. Distinct patterns of antibody responses, by level and IgG subclass distribution, were observed in inhabitants of a malaria-endemic area. Antibodies affinity purified toward each peptide differed in their functional capacity to mediate parasite killing in ADCI assays: 3 of 6 overlapping peptides had a major inhibitory effect on parasite growth. This result was confirmed by the passive transfer of anti-MSP3 antibodies in vivo in a P. falciparum mouse model. T helper cell epitopes were identified in each peptide. Antigenicity and functional assays identified a 70-amino acid conserved domain of MSP3 as a target of biologically active antibodies to be included in future vaccine constructs based on MSP3.
Keywords
Animals Antibodies, Protozoan/*immunology Antigens, Protozoan/*chemistry/genetics/*immunology Disease Models, Animal Drug Design Epitopes, B-Lymphocyte Humans Immunocompromised Host Malaria, Falciparum/*immunology/parasitology Male Mice Plasmodium falciparum/*immunology Protozoan Proteins/*chemistry/genetics/*immunology Protozoan Vaccines Recombinant Proteins/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:55
Last modification date
25/09/2019 6:10
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