Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_CA73769E425F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes.
Journal
Cells
Author(s)
Nicolay W., Moeller R., Kahl S., Vondran FWR, Pietschmann T., Kunz S., Gerold G.
ISSN
2073-4409 (Electronic)
ISSN-L
2073-4409
Publication state
Published
Issued date
04/11/2021
Peer-reviewed
Oui
Volume
10
Number
11
Pages
3019
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes.
Keywords
Carcinoma, Hepatocellular/pathology, Cell Line, Tumor, Cells, Cultured, DEAD Box Protein 58/immunology, Hepatocytes/immunology, Hepatocytes/virology, Humans, Immunity, Innate, Interferons/immunology, Liver/cytology, Liver/immunology, Liver/virology, Liver Neoplasms/pathology, RNA/immunology, RNA Viruses/physiology, Receptors, Immunologic/immunology, Signal Transduction/immunology, Toll-Like Receptor 3/immunology, Viral Load, RIG-I, RNA virus, TLR3, arenavirus, coronavirus, hepatoma cells, innate immunity, interferon, liver, primary hepatocytes
Pubmed
Web of science
Open Access
Yes
Create date
03/12/2021 11:47
Last modification date
08/08/2024 7:40
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