Characterisation of GFAP-Expressing Glial Cells in the Dorsal Root Ganglion after Spared Nerve Injury.
Details
Serval ID
serval:BIB_CA5F9B08C0FA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterisation of GFAP-Expressing Glial Cells in the Dorsal Root Ganglion after Spared Nerve Injury.
Journal
International journal of molecular sciences
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
25/10/2023
Peer-reviewed
Oui
Volume
24
Number
21
Pages
15559
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
Satellite glial cells (SGCs), enveloping primary sensory neurons' somas in the dorsal root ganglion (DRG), contribute to neuropathic pain upon nerve injury. Glial fibrillary acidic protein (GFAP) serves as an SGC activation marker, though its DRG satellite cell specificity is debated. We employed the hGFAP-CFP transgenic mouse line, designed for astrocyte studies, to explore its expression within the peripheral nervous system (PNS) after spared nerve injury (SNI). We used diverse immunostaining techniques, Western blot analysis, and electrophysiology to evaluate GFAP+ cell changes. Post-SNI, GFAP+ cell numbers increased without proliferation, and were found near injured ATF3+ neurons. GFAP+ FABP7+ SGCs increased, yet 75.5% of DRG GFAP+ cells lacked FABP7 expression. This suggests a significant subset of GFAP+ cells are non-myelinating Schwann cells (nmSC), indicated by their presence in the dorsal root but not in the ventral root which lacks unmyelinated fibres. Additionally, patch clamp recordings from GFAP+ FABP7-cells lacked SGC-specific K <sub>ir</sub> 4.1 currents, instead displaying outward K <sub>v</sub> currents expressing K <sub>v</sub> 1.1 and K <sub>v</sub> 1.6 channels specific to nmSCs. In conclusion, this study demonstrates increased GFAP expression in two DRG glial cell subpopulations post-SNI: GFAP+ FABP7+ SGCs and GFAP+ FABP7- nmSCs, shedding light on GFAP's specificity as an SGC marker after SNI.
Keywords
Animals, Mice, Ganglia, Spinal/metabolism, Glial Fibrillary Acidic Protein/genetics, Glial Fibrillary Acidic Protein/metabolism, Neuroglia/metabolism, Satellite Cells, Perineuronal/metabolism, Neuralgia/metabolism, Trauma, Nervous System/metabolism, GFAP, dorsal root ganglion, nerve injury, neuropathic pain, non-myelinating Schwann cells, satellite glial cells
Pubmed
Web of science
Open Access
Yes
Create date
16/11/2023 15:35
Last modification date
19/12/2023 8:26