T6SS contributes to gut microbiome invasion and killing of an herbivorous pest insect by plant-beneficial Pseudomonas protegens.

Details

Ressource 1Request a copy Sous embargo jusqu'au 30/10/2019.
State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_C99CAAA5C3DC
Type
Article: article from journal or magazin.
Collection
Publications
Title
T6SS contributes to gut microbiome invasion and killing of an herbivorous pest insect by plant-beneficial Pseudomonas protegens.
Journal
The ISME Journal
Author(s)
Vacheron J., Péchy-Tarr M., Brochet S., Heiman C.M., Stojiljkovic M., Maurhofer M., Keel C.
ISSN
1751-7370 (Electronic)
ISSN-L
1751-7362
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
13
Number
5
Pages
1318-1329
Language
english
Abstract
Pseudomonas protegens are multi-talented plant-colonizing bacteria that suppress plant pathogens and stimulate plant defenses. In addition, they are capable of invading and killing agriculturally important plant pest insects that makes them promising candidates for biocontrol applications. Here we assessed the role of type VI secretion system (T6SS) components of type strain CHA0 during interaction with larvae of the cabbage pest Pieris brassicae. We show that the T6SS core apparatus and two VgrG modules, encompassing the respective T6SS spikes (VgrG1a and VgrG1b) and associated effectors (RhsA and Ghh1), contribute significantly to insect pathogenicity of P. protegens in oral infection assays but not when bacteria are injected directly into the hemolymph. Monitoring of the colonization levels of P. protegens in the gut, hemolymph, and excrements of the insect larvae revealed that the invader relies on T6SS and VgrG1a module function to promote hemocoel invasion. A 16S metagenomic analysis demonstrated that T6SS-supported invasion by P. protegens induces significant changes in the insect gut microbiome affecting notably Enterobacteriaceae, a dominant group of the commensal gut bacteria. Our study supports the concept that pathogens deploy T6SS-based strategies to disrupt the commensal microbiota in order to promote host colonization and pathogenesis.
Keywords
Ecology, Evolution, Behavior and Systematics, Microbiology
Pubmed
Web of science
Create date
26/01/2019 11:14
Last modification date
20/08/2019 16:44
Usage data