Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_C97FC4EB0859
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects.
Journal
Nutrients
Author(s)
Surowska A., Jegatheesan P., Campos V., Marques A.S., Egli L., Cros J., Rosset R., Lecoultre V., Kreis R., Boesch C., Pouymayou B., Schneiter P., Tappy L.
ISSN
2072-6643 (Electronic)
ISSN-L
2072-6643
Publication state
Published
Issued date
21/01/2019
Peer-reviewed
Oui
Volume
11
Number
1
Language
english
Notes
Publication types: Journal Article ; Randomized Controlled Trial
Publication Status: epublish
Abstract
Sucrose overfeeding increases intrahepatocellular (IHCL) and intramyocellular (IMCL) lipid concentrations in healthy subjects. We hypothesized that these effects would be modulated by diet protein/fat content. Twelve healthy men and women were studied on two occasions in a randomized, cross-over trial. On each occasion, they received a 3-day 12% protein weight maintenance diet (WM) followed by a 6-day hypercaloric high sucrose diet (150% energy requirements). On one occasion the hypercaloric diet contained 5% protein and 25% fat (low protein-high fat, LP-HF), on the other occasion it contained 20% protein and 10% fat (high protein-low fat, HP-LF). IHCL and IMCL concentrations (magnetic resonance spectroscopy) and energy expenditure (indirect calorimetry) were measured after WM, and again after HP-LF/LP-HF. IHCL increased from 25.0 ± 3.6 after WM to 147.1 ± 26.9 mmol/kg wet weight (ww) after LP-HF and from 30.3 ± 7.7 to 57.8 ± 14.8 after HP-LF (two-way ANOVA with interaction: p < 0.001 overfeeding x protein/fat content). IMCL increased from 7.1 ± 0.6 to 8.8 ± 0.7 mmol/kg ww after LP-HF and from 6.2 ± 0.6 to 6.9 ± 0.6 after HP-LF, (p < 0.002). These results indicate that liver and muscle fat deposition is enhanced when sucrose overfeeding is associated with a low protein, high fat diet compared to a high protein, low fat diet.
Keywords
Adipose Tissue/metabolism, Adiposity/drug effects, Adult, Cross-Over Studies, Diet, Diet, High-Fat/adverse effects, Diet, Protein-Restricted/adverse effects, Dietary Fats/administration & dosage, Dietary Fats/metabolism, Dietary Fats/pharmacology, Dietary Proteins/administration & dosage, Dietary Proteins/pharmacology, Dietary Sucrose/adverse effects, Energy Intake, Feeding Behavior, Female, Healthy Volunteers, Humans, Lipid Metabolism/drug effects, Lipids, Liver/cytology, Liver/drug effects, Liver/metabolism, Male, Muscle, Skeletal/cytology, Muscle, Skeletal/drug effects, Muscle, Skeletal/metabolism, Young Adult, dietary fat content, dietary protein content, energy expenditure, hepatic steatosis, intrahepatocellular lipids, intramyocellular lipids, plasma triglyceride, sucrose overfeeding
Pubmed
Web of science
Open Access
Yes
Create date
17/02/2019 16:20
Last modification date
22/10/2019 6:11
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