Cryopreservation of starfish oocytes.

Details

Serval ID
serval:BIB_C92DE31EB8E6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cryopreservation of starfish oocytes.
Journal
Cryobiology
Author(s)
Hamaratoğlu F., Eroğlu A., Toner M., Sadler K.C.
ISSN
0011-2240 (Print)
ISSN-L
0011-2240
Publication state
Published
Issued date
2005
Volume
50
Number
1
Pages
38-47
Language
english
Abstract
Research from many laboratories over the past several decades indicates that invertebrate oocytes and eggs are extraordinarily difficult to freeze. Since starfish oocytes, eggs, and embryos are an important cell and developmental biology model system, there is great interest to cryopreserve these cells. Previous starfish oocyte cryopreservation studies using slow cooling protocols revealed that these cells are highly sensitive to osmotic stress and form intracellular ice at very high sub-zero temperatures, suggesting that common freezing methodologies may not prove useful. We report here that a short exposure to 1.5 M Me2SO/1 M trehalose in hypotonic salt solution followed by ultra-rapid cooling to cryogenic temperatures allows starfish oocytes to be cryopreserved with the average survival rate of 34% when normalized to control oocytes that were exposed to CPA, but not frozen. On average, 51% of the oocytes in 77% of the batches of frozen oocytes underwent meiotic maturation in response to the starfish maturation hormone, 1-methyladenine. In one experiment, eggs developing from thawed oocytes were capable of being fertilized and two developed into embryos. These data suggests that successful cryopreservation of starfish oocytes is possible, but will need further refinement to increase the numbers of fully competent embryos.
Keywords
Adenine/analogs & derivatives, Adenine/chemistry, Animals, Cell Survival, Cryopreservation/methods, Cryoprotective Agents/pharmacology, Culture Techniques, Female, Freezing, Ice, Image Processing, Computer-Assisted, Meiosis, Oocytes/cytology, Oocytes/metabolism, Osmosis, Preservation, Biological, Starfish, Temperature, Trehalose/pharmacology
Pubmed
Web of science
Create date
12/02/2014 16:42
Last modification date
20/08/2019 15:44
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