Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells
Details
Serval ID
serval:BIB_C8CB950639E5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells
Journal
American Journal of Physiology
ISSN
0363-6127
Publication state
Published
Issued date
06/1989
Volume
256
Number
6 Pt 2
Pages
F1034-43
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Polyclonal antibodies raised against the alpha- and beta-subunits of amphibian kidney Na+-K+-ATPase were used to screen an expression library from Xenopus laevis kidney epithelial cells (A6 cell line). cDNAs coding for each Na+-K+-ATPase subunit were identified and used to isolate near full-length cDNAs. The complete nucleotide sequence and the deduced amino acid sequence were determined. The alpha-subunit is an alpha (alpha I)-isoform. The alpha- and beta-subunits are more closely related to the mammalian and avian than the fish sequences. Antibodies raised against the fusion proteins produced by the two clones served to immunoprecipitate proteins from biosynthetically labeled or selectively surface-radioiodinated A6 cells grown on a porous substrate. The alpha- and the beta-subunits of Na+-K+-ATPase were found associated early in the course of biosynthesis and were restricted to the basolateral plasma membrane.
Keywords
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cloning, Molecular
DNA/genetics
Immunoenzyme Techniques
Kidney/*enzymology
Macromolecular Substances
Molecular Sequence Data
Na(+)-K(+)-Exchanging ATPase/*genetics
Restriction Mapping
Sequence Homology, Nucleic Acid
Species Specificity
Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 13:28
Last modification date
20/08/2019 16:43