Analysis of human chorionic gonadotropin (hCG): application of routine immunological methods for initial testing and confirmation analysis in doping control.
Details
Serval ID
serval:BIB_C8C99F12F903
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of human chorionic gonadotropin (hCG): application of routine immunological methods for initial testing and confirmation analysis in doping control.
Journal
Drug testing and analysis
ISSN
1942-7611 (Electronic)
ISSN-L
1942-7603
Publication state
Published
Issued date
08/2013
Peer-reviewed
Oui
Volume
5
Number
8
Pages
614-618
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Human chorionic gonadotropin (hCG) is dimeric glycoprotein produced by placenta in pregnancy and also in low levels by pituitary gland. The main clinical use for exogenous hCG-administration is typically linked to infertility. The desired effect of hCG misuse in sport is due to the enhancement of testicular production of testosterone. Therefore, hCG is listed by the World Anti-Doping Agency (WADA) as a prohibited substance in male athletes and according to the recently published WADA guideline urinary concentrations of hCG > 5 IU/L may be an indicator of doping. In this study two independent immunoassays were used to implement the new WADA guideline. The assay for initial testing (Siemens Immulite 2000 XPi hCG assay) recognizes various hCG variants (e.g. hCG and β-core fragment of hCG) whereas the confirmatory assay (PerkinElmer DELFIA Xpress hCG) is sensitive to intact and nicked hCG only. Both assays showed adequate sensitivity and were proven fit-for-purpose in routine doping control. Population-based distribution of the assays was in good agreement with results of earlier studies and supported well the current threshold of 5 IU/L.
Keywords
Chorionic Gonadotropin/urine, Chorionic Gonadotropin, beta Subunit, Human/urine, Doping in Sports, Humans, Immunoassay/methods, Male, Peptide Fragments/urine, Reproducibility of Results, Sensitivity and Specificity, Substance Abuse Detection/methods
Pubmed
Web of science
Create date
02/05/2017 16:05
Last modification date
20/08/2019 16:43