Genomic characterisation of an international Pseudomonas aeruginosa reference panel indicates that the two major groups draw upon distinct mobile gene pools.

Details

Serval ID
serval:BIB_C8AD256CBA60
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genomic characterisation of an international Pseudomonas aeruginosa reference panel indicates that the two major groups draw upon distinct mobile gene pools.
Journal
FEMS microbiology letters
Author(s)
Freschi L., Bertelli C., Jeukens J., Moore M.P., Kukavica-Ibrulj I., Emond-Rheault J.G., Hamel J., Fothergill J.L., Tucker N.P., McClean S., Klockgether J., de Soyza A., Brinkman FSL, Levesque R.C., Winstanley C.
ISSN
1574-6968 (Electronic)
ISSN-L
0378-1097
Publication state
Published
Issued date
01/07/2018
Peer-reviewed
Oui
Volume
365
Number
14
Pages
1
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Pseudomonas aeruginosa is an important opportunistic pathogen, especially in the context of infections of cystic fibrosis (CF). In order to facilitate coordinated study of this pathogen, an international reference panel of P. aeruginosa isolates was assembled. Here we report the genome sequencing and analysis of 33 of these isolates and 7 reference genomes to further characterise this panel. Core genome single nucleotide variant phylogeny demonstrated that the panel strains are widely distributed amongst the P. aeruginosa population. Common loss-of-function mutations reported as adaptive during CF (such as in mucA and mexA) were identified amongst isolates from chronic respiratory infections. From the 40 strains analysed, 37 unique resistomes were predicted, based on the Resistance Gene Identifier method using the Comprehensive Antibiotic Resistance Database. Notably, hierarchical clustering and phylogenetic reconstructions based on the presence/absence of genomic islands (GIs), prophages and other regions of genome plasticity (RGPs) supported the subdivision of P. aeruginosa into two main groups. This is the largest, most diverse analysis of GIs and associated RGPs to date, and the results suggest that, at least at the largest clade grouping level (group 1 vs group 2), each group may be drawing upon distinct mobile gene pools.
Keywords
Adaptation, Physiological/genetics, Cystic Fibrosis/microbiology, Drug Resistance, Microbial/genetics, Genes, Bacterial/genetics, Genome, Bacterial/genetics, Genomic Islands/genetics, Genomics, Humans, Mutation, Phylogeny, Polymorphism, Single Nucleotide, Prophages/genetics, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/classification, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/isolation & purification, Pseudomonas aeruginosa/physiology, Sequence Analysis, DNA
Pubmed
Web of science
Create date
25/06/2018 9:50
Last modification date
09/10/2019 6:09
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