Liver-directed gene transfer: a linear polyethlenimine derivative mediates highly efficient DNA delivery to primary hepatocytes in vitro and in vivo

Details

Serval ID
serval:BIB_C877C4DE5FEF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Liver-directed gene transfer: a linear polyethlenimine derivative mediates highly efficient DNA delivery to primary hepatocytes in vitro and in vivo
Journal
Journal of Viral Hepatitis
Author(s)
Chemin  I., Moradpour  D., Wieland  S., Offensperger  W. B., Walter  E., Behr  J. P., Blum  H. E.
ISSN
1352-0504 (Print)
Publication state
Published
Issued date
11/1998
Volume
5
Number
6
Pages
369-75
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Efficient DNA delivery is a prerequisite for the successful implementation of molecular antiviral strategies against chronic viral hepatitis and gene therapy in general. The cationic polymer polyethylenimine (PEI) has recently been explored as a gene transfer vector in various cell types in vitro and in vivo. In this study, we evaluated a linear PEI derivative (lPEI) as a vector for gene and oligodeoxynucleotide transfer into hepatocytes in vitro and in vivo. A simple protocol was developed that allowed transfection of up to 50% of primary hepatocytes in vitro. In addition, fluorescent oligodeoxynucleotides were efficiently delivered to the liver in vivo after intravenous injection into Pekin ducks. Thus, lPEI mediates highly efficient gene and oligodeoxynucleotide transfer into primary hepatocytes and is potentially useful for DNA delivery in vivo.
Keywords
Animals Cells, Cultured DNA/*administration & dosage Ducks Gene Therapy/methods *Gene Transfer Techniques *Genetic Vectors Liver/*cytology Microscopy, Fluorescence Oligodeoxyribonucleotides, Antisense/administration & dosage Polyethyleneimine/*analogs & derivatives Transfection
Pubmed
Web of science
Create date
25/01/2008 17:06
Last modification date
20/08/2019 16:43
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