Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing
Details
Serval ID
serval:BIB_C859FD51D4B3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing
Journal
Nature Structural Biology
ISSN
1072-8368 (Print)
Publication state
Published
Issued date
01/1998
Volume
5
Number
1
Pages
31-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Abstract
Several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an internal protein (intein). Post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external protein (extein) and an intein. Most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. We report here the crystal structure of an intein found in the gyrase A subunit from Mycobacterium xenopi at 2.2 A resolution. The structure contains an unusual beta-fold with the catalytic splice junctions at the ends of two adjacent antiparallel beta-strands. The arrangement of the active site residues Ser 1, Thr 72, His 75, His 197, and Asn 198 is consistent with a four-step mechanism for the cleavage-ligation reaction. Using site-directed mutagenesis, the N-terminal cysteine, proposed as the nucleophile in the first step of the splicing reaction, was changed to a Ser 1 and Ala 0, thus capturing the intein in a pre-spliced state.
Keywords
Amino Acid Sequence
DNA Gyrase
DNA Topoisomerases, Type II/*metabolism/ultrastructure
Deoxyribonucleases, Type II Site-Specific/metabolism/ultrastructure
*Drosophila Proteins
Endonucleases/chemistry/metabolism
Insect Proteins/chemistry
Molecular Sequence Data
Mycobacterium xenopi
Protein Precursors/metabolism
*Protein Splicing
Protein Structure, Tertiary
Saccharomyces cerevisiae
Pubmed
Web of science
Create date
25/01/2008 15:45
Last modification date
20/08/2019 16:43