Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants.

Details

Serval ID
serval:BIB_C8411F5FDCB5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants.
Journal
eLife
Author(s)
Arguello J.R., Abuin L., Armida J., Mika K., Chai P.C., Benton R.
ISSN
2050-084X (Electronic)
ISSN-L
2050-084X
Publication state
Published
Issued date
05/03/2021
Peer-reviewed
Oui
Volume
10
Pages
e63036
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Determining the molecular properties of neurons is essential to understand their development, function and evolution. Using Targeted DamID (TaDa), we characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic receptor (Ir)-expressing olfactory sensory neurons in Drosophila. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not consistently associated with differences in chromatin accessibility, but rather to distinct transcription factor profiles. Genes that are heterogeneously expressed across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration.
Keywords
D. melanogaster, developmental biology, neuroscience, neuron, olfaction, transcriptomics
Pubmed
Web of science
Open Access
Yes
Create date
10/07/2020 11:33
Last modification date
24/04/2021 6:33
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