Carbon and nitrogen stable isotope variations in leaves of two grapevine cultivars (Chasselas and Pinot noir): Implications for ecophysiological studies.

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State: Public
Version: author
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_C810E559E134
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Carbon and nitrogen stable isotope variations in leaves of two grapevine cultivars (Chasselas and Pinot noir): Implications for ecophysiological studies.
Journal
Plant physiology and biochemistry
Author(s)
Spangenberg J. E., Schweizer M., Zufferey V.
ISSN
1873-2690 (Electronic)
ISSN-L
0981-9428
Publication state
Published
Issued date
06/2021
Peer-reviewed
Oui
Volume
163
Pages
45-54
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
We investigated the within- and between-leaf variability in the carbon and nitrogen isotope composition (δ <sup>13</sup> C and δ <sup>15</sup> N) and total nitrogen (TN) content in two grapevine cultivars (Vitis vinifera cv. Chasselas and Pinot noir) field-grown under rain-fed conditions. The within-leaf variability was studied in discs sampled from base-to-tip and left and right regions from the margin to midrib. The intra- and interplant variability was studied by comparing leaves at different positions along the shoot (basal, median, apical). In leaves from both cultivars, a decrease in δ <sup>13</sup> C from base to tip was observed, which is in line with an upward gradient of stomatal density and chlorophyll concentration. Less important, but still significant differences were observed between the right and left discs. The leaf TN and δ <sup>15</sup> N values differed between cultivars, showed smaller variations than the δ <sup>13</sup> C values, and no systematic spatial trends. The intraleaf variations in δ <sup>13</sup> C, δ <sup>15</sup> N, and TN suggest that stomatal behavior, CO <sub>2</sub> fixation, chlorophyll concentrations, and the chemical composition of leaf components were heterogeneous in the leaves. At the canopy scale, the apical leaves had less <sup>13</sup> C and more <sup>15</sup> N and TN than the basal leaves, indicating differences in their photosynthetic capacity and remobilizations from old, senescing leaves to younger leaves. Overall, this study demonstrates patchiness in the δ <sup>13</sup> C and δ <sup>15</sup> N values of grapevine leaves and species-specificity of the nitrogen assimilation and <sup>15</sup> N fractionation. These findings suggest that care must be taken not to overinterpret foliar δ <sup>13</sup> C and δ <sup>15</sup> N values in studies based on fragmented material as markers of physiological and biochemical responses to environmental factors.
Keywords
Carbon, Carbon Isotopes, Nitrogen, Nitrogen Isotopes, Plant Leaves, Intraleaf variations, Intraplant variations, Leaf Discs, Leaf patchiness, Leaf total nitrogen, Leaf δ(13)C and δ(15)N
Pubmed
Web of science
Open Access
Yes
Create date
01/06/2021 9:17
Last modification date
28/06/2021 6:35
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