A singular bacteriophytochrome acquired by lateral gene transfer.
Details
Serval ID
serval:BIB_C80B9F880632
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A singular bacteriophytochrome acquired by lateral gene transfer.
Journal
Journal of Biological Chemistry
ISSN
0021-9258
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
282
Number
10
Pages
7320-7328
Language
english
Notes
Publication types: Journal Article
Abstract
Bacteriophytochromes are phytochrome-like proteins that mediate photosensory responses in various bacteria according to their light environment. The genome of the photosynthetic and plant-symbiotic Bradyrhizobium sp. strain ORS278 revealed the presence of a genomic island acquired by lateral transfer harboring a bacteriophytochrome gene, BrBphP3.ORS278, and genes involved in the synthesis of phycocyanobilin and gas vesicles. The corresponding protein BrBphP3.ORS278 is phylogenetically distant from the other (bacterio)phytochromes described thus far and displays a series of unusual properties. It binds phycocyanobilin as a chromophore, a unique feature for a bacteriophytochrome. Moreover, its C-terminal region is short and displays no homology with any known functional domain. Its dark-adapted state absorbs maximally around 610 nm, an unusually short wavelength for (bacterio)phytochromes. This form is designated as Po for orange-absorbing form. Upon illumination, a photo-reversible switch occurs between the Po form and a red (670 nm)-absorbing form (Pr), which rapidly backreacts in the dark. Because of this instability, illumination results in a mixture of the Po and Pr states in proportions that depend on the intensity. These uncommon features suggest that BrBphP3.ORS278 could be fitted to measure light intensity rather than color.
Keywords
Amino Acid Sequence, Bacterial Proteins/chemistry, Bacterial Proteins/genetics, Bradyrhizobium/genetics, Fluorescence, Gene Transfer, Horizontal, Molecular Sequence Data, Phycobilins/metabolism, Phycocyanin/metabolism, Phytochrome/chemistry, Phytochrome/genetics, Temperature
Pubmed
Web of science
Open Access
Yes
Create date
13/03/2008 11:24
Last modification date
20/08/2019 16:43