Establishment of two rabbit mammary epithelial cell lines with distinct oncogenic potential and differentiated phenotype after microinjection of transforming genes

Details

Serval ID
serval:BIB_C79CA0353842
Type
Article: article from journal or magazin.
Collection
Publications
Title
Establishment of two rabbit mammary epithelial cell lines with distinct oncogenic potential and differentiated phenotype after microinjection of transforming genes
Journal
Molecular and Cellular Biology
Author(s)
Garcia  I., Sordat  B., Rauccio-Farinon  E., Dunand  M., Kraehenbuhl  J. P., Diggelmann  H.
ISSN
0270-7306 (Print)
Publication state
Published
Issued date
06/1986
Volume
6
Number
6
Pages
1974-82
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
The goal of this work was to establish an assay for transformation of epithelial cells. Two epithelial cell lines were obtained after microinjecting transforming genes into primary rabbit mammary secretory cells. The cell lines were analyzed for their oncogenic potential and for the maintenance of a differentiated phenotype. A fully transformed cell line, which retained epithelial cell organization, was obtained by coinjecting simian virus 40 DNA and the activated human c-Ha-ras gene. The proliferation rate of these cells was high, with a doubling time of 16 h. Their growth was anchorage independent, and they had lost contact inhibition. The cells were tumorigenic in nude mice, but had no metastatic potential. Both microinjected DNAs were efficiently transcribed and translated, in contrast to the casein genes, which were expressed in primary cells but not in the transformed cell line. An immortalized cell line established after injection with simian virus 40 DNA alone was characterized by a moderate rate of proliferation with a doubling time of approximately 30 h. The growth of these cells was contact inhibited and anchorage dependent. The cells were not tumorigenic in nude mice. The viral DNA was expressed during early passages, as shown by the presence of the large T antigen in cell nuclei, but not at later passages. A high number of lactogenic hormone receptors were found associated with the cell surface. Despite the presence of these receptors, no induction of genes coding for milk proteins was observed after addition of prolactin. These data demonstrate that this assay system can be used to assess the immortalizing and transforming potential of candidate oncogenes in epithelial cells.
Keywords
Animals Caseins/genetics Cell Differentiation Cell Line *Cell Transformation, Viral DNA, Neoplasm/genetics DNA, Viral/genetics Epithelial Cells Gene Expression Regulation Mammary Glands, Animal/*cytology Microscopy, Electron Neoplasms, Experimental/genetics *Oncogenes Rabbits Simian virus 40
Pubmed
Web of science
Create date
25/01/2008 16:05
Last modification date
03/03/2018 21:19
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