Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.

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State: Serval
Version: author
Serval ID
serval:BIB_C6CE9D9DBA79
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.
Journal
Fems Microbiology Reviews
Author(s)
Croxatto A., Prod'hom G., Greub G.
ISSN
1574-6976 (Electronic)
ISSN-L
0168-6445
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
36
Number
2
Pages
380-407
Language
english
Abstract
Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.
Keywords
Animals, Bacteria/chemistry, Bacteria/isolation & purification, Bacterial Infections/diagnosis, Bacterial Infections/microbiology, Diagnostic Techniques and Procedures, Fungi/chemistry, Fungi/isolation & purification, Humans, Mycoses/diagnosis, Mycoses/microbiology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/economics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Pubmed
Web of science
Open Access
Yes
Create date
18/02/2012 13:36
Last modification date
09/05/2019 1:04
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