Differential regulation of Na-K-ATPase isoform gene expression by T3 during rat brain development.

Details

Serval ID
serval:BIB_C699C5580A10
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential regulation of Na-K-ATPase isoform gene expression by T3 during rat brain development.
Journal
American Journal of Physiology. Cell Physiology
Author(s)
Corthésy-Theulaz I., Mérillat A.M., Honegger P., Rossier B.C.
ISSN
0363-6143
ISSN-L
0002-9513
Publication state
Published
Issued date
1991
Volume
261
Number
1 Pt 1
Pages
C124-C131
Language
english
Abstract
A fetal rat telencephalon organotypic cell culture system was found to reproduce the developmental pattern of Na-K-adenosinetriphosphatase (ATPase) gene expression observed in vivo [Am. J. Physiol. 258 (Cell Physiol. 27): C1062-C1069, 1990]. We have used this culture system to study the effects of triiodothyronine (T3; 0.003-30 nM) on mRNA abundance and basal transcription rates of Na-K-ATPase isoforms. Steady-state mRNA levels were low at culture day 6 (corresponding to the day of birth) but distinct for each isoform alpha 3 much greater than beta 1 = beta 2 greater than alpha 2 greater than alpha 1. At culture day 6, T3 did not modify mRNA abundance of any isoform. At culture day 12 (corresponding to day 7 postnatal), T3 increased the mRNA level of alpha 2 (4- to 7-fold), beta 2 (4- to 5-fold), alpha 1 (3- to 6-fold), and beta 1 (1.5-fold), whereas alpha 3 mRNA levels remained unchanged. Interestingly, the basal transcription rate for each isoform differed strikingly (alpha 2 greater than alpha 1 much greater than beta 1 = beta 2 greater than alpha 3) but remained stable throughout 12 days of culture and was not regulated by T3. Thus we observed an inverse relationship between rate of transcription and rate of mRNA accumulation for each alpha-isoform, suggesting that alpha 1- and alpha 2-mRNA are turning over rapidly whereas alpha 3-mRNA is turning over slowly. Our data indicate that one of the mechanisms by which T3 selectively controls Na-K-ATPase gene expression during brain development in vitro occurs at the posttranscriptional level.
Keywords
Animals, Blotting, Northern, Cells, Cultured, Gene Expression Regulation, Enzymologic, Immunoblotting, Isoenzymes/biosynthesis, Isoenzymes/genetics, Kinetics, RNA, Messenger/analysis, Rats, Sodium-Potassium-Exchanging ATPase/biosynthesis, Sodium-Potassium-Exchanging ATPase/genetics, Telencephalon/embryology, Telencephalon/enzymology, Transcription, Genetic, Triiodothyronine/physiology
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 15:42
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