Differentially expressed nucleolar transforming growth factor-beta1 target (DENTT) exhibits an inhibitory role on tumorigenesis.

Details

Serval ID
serval:BIB_C5D2F664B95E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differentially expressed nucleolar transforming growth factor-beta1 target (DENTT) exhibits an inhibitory role on tumorigenesis.
Journal
Carcinogenesis
Author(s)
Kandalaft L.E., Zudaire E., Portal-Núñez S., Cuttitta F., Jakowlew S.B.
ISSN
1460-2180 (Electronic)
ISSN-L
0143-3334
Publication state
Published
Issued date
06/2008
Peer-reviewed
Oui
Volume
29
Number
6
Pages
1282-1289
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Intramural
Publication Status: ppublish
Abstract
Differentially expressed nucleolar transforming growth factor-beta1 target (DENTT), also known as testis-specific protein Y-encoded-like (TSPYL-2) and cell division autoantigen-1, is a member of the testis-specific protein Y-encoded (TSPY)/TSPY-L/SET/nucleosome assembly protein-1 superfamily. DENTT is expressed in various tissues including normal human lung. Here, we investigate the involvement of DENTT in cancer promotion and progression. DENTT messenger RNA (mRNA) and protein levels were shown to be markedly downregulated in human and mouse primary tumors and in human tumor cell lines. Overexpression of DENTT in human lung (A549-DENTT) and breast (MCF-7-DENTT) cancer cells resulted in diminished growth potential in anchorage-dependent growth assays and reduced capacity to form colonies under anchorage-independent culture conditions. The migratory potential of A549-DENTT and MCF-7-DENTT cells was reduced when compared with empty vector control cells. Treating human lung cell lines with demethylating agents increased DENTT expression significantly. DENTT expression pattern paralleled that of transforming growth factor-beta1 (TGF-beta1) in normal and malignant tissue and ectopic expression or treatment with TGF-beta1 in lung cancer cells was followed by increased DENTT mRNA and protein levels. Collectively, our results suggest a role for DENTT as a suppressor of the tumorigenic phenotype.

Keywords
Animals, Blotting, Western, Breast Neoplasms/metabolism, Cell Line, Tumor, Cell Movement/physiology, Cell Proliferation, Enzyme-Linked Immunosorbent Assay, Female, Gene Expression, Gene Expression Regulation, Humans, Immunohistochemistry, Lung Neoplasms/metabolism, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, RNA, Messenger/analysis, Reverse Transcriptase Polymerase Chain Reaction, Transfection, Transforming Growth Factor beta1/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
09/02/2018 15:59
Last modification date
20/08/2019 16:41
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