Intraocular pressure in Lewis rats

Details

Serval ID
serval:BIB_C53FECA486F5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Intraocular pressure in Lewis rats
Journal
Investigative Ophthalmology and Visual Science
Author(s)
Mermoud  A., Baerveldt  G., Minckler  D. S., Lee  M. B., Rao  N. A.
ISSN
0146-0404 (Print)
Publication state
Published
Issued date
04/1994
Volume
35
Number
5
Pages
2455-60
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Abstract
PURPOSE. To perform noninvasive measurements of intraocular pressure (IOP) in rats, the Tono-Pen-1 and Tono-Pen-2 were calibrated against direct manometry. Normal values and the long-term fluctuations of IOP in Lewis rats were established. METHODS. For calibration, 24 eyes were cannulated and connected to a pressure transducer with a chart recorder. IOP was increased from 5 to 40 mm Hg in 5 mm Hg increments, and from 40 to 60 mm Hg in 10 mm Hg increments. After each incremental increase, IOP was measured with a Tono-Pen-1 and a Tono-Pen-2 tonometer. To determine normal IOP in Lewis rats, IOP was measured with a Tono-Pen-1 in 229 eyes of 115 rats, and a histogram of normal IOP was established. To ascertain long-term IOP fluctuations, the pressure in 52 eyes of 26 rats was measured every day between 8:30 and 9:30 AM for 7 consecutive days. RESULTS. Plotting the mean Tono-Pen readings for each eye against the transducer IOP produced two regression formulas: y = 1.819 + 0.711 x (r2 = 0.92) for Tono-Pen-1, and y = -1.291 + 0.784 x (r2 = 0.97) for Tono-Pen-2. The normal IOP in rats was 17.30 +/- 5.25 mm Hg (90% confidence interval: 7.28 and 26.98 mm Hg for the lower and upper limits of normal IOP). There was no long-term fluctuation in IOP (P = 0.55). CONCLUSIONS. IOP can be measured accurately in living rats with the Tono-Pen-1 or the Tono-Pen-2.
Keywords
Animals Calibration Intraocular Pressure/*physiology Longitudinal Studies Manometry Rats Rats, Inbred Lew Reference Values Tonometry, Ocular/methods
Pubmed
Web of science
Create date
28/01/2008 13:50
Last modification date
20/08/2019 16:40
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