Circular polymerization of the membranolytic ninth component of complement. Dependence on metal ions.
Details
Serval ID
serval:BIB_C52D84435A55
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Circular polymerization of the membranolytic ninth component of complement. Dependence on metal ions.
Journal
Journal of Biological Chemistry
ISSN
0021-9258
Publication state
Published
Issued date
08/1984
Peer-reviewed
Oui
Volume
259
Number
16
Pages
10569-10573
Language
english
Abstract
The ninth component of complement (C9) polymerizes to tubules either spontaneously at a very slow rate (3 days at 37 degrees C) or within minutes if induced by C5b-8 as part of the membrane attack assembly (Tschopp, J., Podack, E. R., and Müller-Eberhard, H. J. (1984) J. Immunol., in press). The spontaneous formation of tubular sodium dodecyl sulfate-resistant C9 polymers was completely inhibited in the presence of 1 mM EDTA. In contrast, the presence of Cu2+, Zn2+, and Cd2+ accelerated C9 polymerization. Zn2+ was the most efficient metal ion. At a molar ratio of one Zn2+ per monomeric C9, all C9 polymerized within 2 h. At Zn2+ concentrations above 10(-3) M, tubular C9 formation was inhibited due to nonspecific aggregation of C9. It is concluded that the presence of metal ions is required for the formation of tubular poly C9 and that Zn2+ in particular catalyzes C9 polymerization.
Keywords
Cadmium/pharmacology, Cations, Divalent, Complement C9/metabolism, Copper/pharmacology, Edetic Acid/pharmacology, Humans, Kinetics, Macromolecular Substances, Microscopy, Electron, Protein Conformation, Zinc/pharmacology
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 15:40