Gene expression during larval caste determination and differentiation in intermediately eusocial bumblebees, and a comparative analysis with advanced eusocial honeybees.

Details

Serval ID
serval:BIB_C48D4F61F58F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Gene expression during larval caste determination and differentiation in intermediately eusocial bumblebees, and a comparative analysis with advanced eusocial honeybees.
Journal
Molecular ecology
Author(s)
Collins D.H., Wirén A., Labédan M., Smith M., Prince D.C., Mohorianu I., Dalmay T., Bourke AFG
ISSN
1365-294X (Electronic)
ISSN-L
0962-1083
Publication state
Published
Issued date
02/2021
Peer-reviewed
Oui
Volume
30
Number
3
Pages
718-735
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The queen-worker caste system of eusocial insects represents a prime example of developmental polyphenism (environmentally-induced phenotypic polymorphism) and is intrinsic to the evolution of advanced eusociality. However, the comparative molecular basis of larval caste determination and subsequent differentiation in the eusocial Hymenoptera remains poorly known. To address this issue within bees, we profiled caste-associated gene expression in female larvae of the intermediately eusocial bumblebee Bombus terrestris. In B. terrestris, female larvae experience a queen-dependent period during which their caste fate as adults is determined followed by a nutrition-sensitive period also potentially affecting caste fate but for which the evidence is weaker. We used mRNA-seq and qRT-PCR validation to isolate genes differentially expressed between each caste pathway in larvae at developmental stages before and after each of these periods. We show that differences in gene expression between caste pathways are small in totipotent larvae, then peak after the queen-dependent period. Relatively few novel (i.e., taxonomically-restricted) genes were differentially expressed between castes, though novel genes were significantly enriched in late-instar larvae in the worker pathway. We compared sets of caste-associated genes in B. terrestris with those reported from the advanced eusocial honeybee, Apis mellifera, and found significant but relatively low levels of overlap of gene lists between the two species. These results suggest both the existence of low numbers of shared toolkit genes and substantial divergence in caste-associated genes between Bombus and the advanced eusocial Apis since their last common eusocial ancestor.
Keywords
Animals, Bees/genetics, Behavior, Animal, Female, Gene Expression, Gene Expression Profiling, Larva/genetics, Bombus terrestris, caste determination, caste-associated gene expression, eusociality, larval development, mRNA-seq
Pubmed
Web of science
Open Access
Yes
Create date
26/11/2020 22:59
Last modification date
09/12/2023 8:03
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