Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease.

Details

Serval ID
serval:BIB_C47C605FFBCF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease.
Journal
Nature medicine
Author(s)
Harari A., Rozot V., Bellutti Enders F., Perreau M., Stalder J.M., Nicod L.P., Cavassini M., Calandra T., Blanchet C.L., Jaton K., Faouzi M., Day C.L., Hanekom W.A., Bart P.A., Pantaleo G.
ISSN
1546-170X (Electronic)
ISSN-L
1078-8956
Publication state
Published
Issued date
03/2011
Peer-reviewed
Oui
Volume
17
Number
3
Pages
372-376
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Rapid diagnosis of active Mycobacterium tuberculosis (Mtb) infection remains a clinical and laboratory challenge. We have analyzed the cytokine profile (interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2)) of Mtb-specific T cells by polychromatic flow cytometry. We studied Mtb-specific CD4+ T cell responses in subjects with latent Mtb infection and active tuberculosis disease. The results showed substantial increase in the proportion of single-positive TNF-α Mtb-specific CD4+ T cells in subjects with active disease, and this parameter was the strongest predictor of diagnosis of active disease versus latent infection. We validated the use of this parameter in a cohort of 101 subjects with tuberculosis diagnosis unknown to the investigator. The sensitivity and specificity of the flow cytometry-based assay were 67% and 92%, respectively, the positive predictive value was 80% and the negative predictive value was 92.4%. Therefore, the proportion of single-positive TNF-α Mtb-specific CD4+ T cells is a new tool for the rapid diagnosis of active tuberculosis disease.

Keywords
CD4-Positive T-Lymphocytes/immunology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Mycobacterium tuberculosis/immunology, Tuberculosis/immunology, Tumor Necrosis Factor-alpha/immunology
Pubmed
Web of science
Create date
31/03/2011 13:41
Last modification date
20/08/2019 16:39
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