Polypeptide marker and disease patterns found while mapping proteins in ascitis

Details

Serval ID
serval:BIB_C475068EB609
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Polypeptide marker and disease patterns found while mapping proteins in ascitis
Journal
Journal of Chromatography
Author(s)
Toussi  A., Paquet  N., Huber  O., Frutiger  S., Tissot  J. D., Hughes  G. J., Hochstrasser  D. F.
ISSN
0021-9673 (Print)
Publication state
Published
Issued date
11/1992
Volume
582
Number
1-2
Pages
87-92
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 6
Abstract
To assess the protein composition of ascitis, 28 samples of ascitic fluid were obtained from patients admitted to Geneva University Hospital. The samples were analysed randomly and blindly by high-resolution two-dimensional polyacrylamide gel electrophoresis. The final visual evaluation was compared with the discharged summary and diagnosis. The protein pattern of ascitis was, as expected, very similar to normal or diseased plasma, with the exception of two spots which were present in ascitic fluids but not in the 200 plasma samples analyzed in parallel. After microsequencing, they proved to be beta-fibrinogen fragments. Several diseases showed distinct patterns, especially acute pancreatitis. A group of intense spots with an apparent molecular mass between those of alpha 1-antitrypsin and beta-haptoglobin were found in all ascitic fluid from pancreatitis cases (six patients). These spots had isoelectric points similar to those of alpha 1-antitrypsin and beta-haptoglobin and microsequencing revealed that they were three different fragments of alpha 1-antitrypsin.
Keywords
Acute Disease Ascites/*metabolism/pathology Biological Markers Electrophoresis, Polyacrylamide Gel Fibrinogen/analysis/metabolism Haptoglobins/analysis/metabolism Humans Pancreatitis/*diagnosis/metabolism/pathology *Peptide Mapping Peptides/*analysis/metabolism alpha 1-Antitrypsin/analysis/metabolism
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 15:39
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