X-ray phase contrast imaging of metallized and non-metallized biological samples.

Details

Serval ID
serval:BIB_C40022B0AB01
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
X-ray phase contrast imaging of metallized and non-metallized biological samples.
Journal
Zeitschrift für Medizinische Physik
Author(s)
Monnin P., Hlushchuk R., Djonov V., Meuli R., Valley J.F., Verdun F.R.
ISSN
0939-3889
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
16
Number
3
Pages
172-178
Language
english
Abstract
Imaging of biological samples has been performed with a variety of techniques for example electromagnetic waves, electrons, neutrons, ultrasound and X-rays. Also conventional X-ray imaging represents the basis of medical diagnostic imaging, it remains of limited use in this application because it is based solely on the differential absorption of X-rays by tissues. Coherent and bright photon beams, such as those produced by third-generation synchrotron X-ray sources, provide further information on subtle X-ray phase changes at matter interfaces. This complements conventional X-ray absorption by edge enhancement phenomena. Thus, phase contrast imaging has the potential to improve the detection of structures on images by detecting those structures that are invisible with X-ray absorption imaging. Images of a weakly absorbing nylon fibre were recorded in in-line holography geometry using a high resolution low-noise CCD camera at the ESRF in Grenoble. The method was also applied to improve image contrast for images of biological tissues. This paper presents phase contrast microradiographs of vascular tree casts and images of a housefly. These reveal very fine structures, that remain invisible with conventional absorption contrast only.
Keywords
Animals, Aortography, Holography/methods, Houseflies, Humans, Image Processing, Computer-Assisted, Metals/analysis, Mice, Microscopy, Phase-Contrast, Radiographic Image Enhancement, Sensitivity and Specificity, Synchrotrons, X-Rays
Pubmed
Web of science
Create date
29/02/2008 17:45
Last modification date
20/08/2019 15:39
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