Spatio-temporal overview of neuroinflammation in an experimental mouse stroke model.

Details

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_C3F547344B91
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatio-temporal overview of neuroinflammation in an experimental mouse stroke model.
Journal
Scientific reports
Author(s)
Buscemi L., Price M., Bezzi P., Hirt L.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
24/01/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
507
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
After ischemic stroke, in the lesion core as well as in the ischemic penumbra, evolution of tissue damage and repair is strongly affected by neuroinflammatory events that involve activation of local specialized glial cells, release of inflammatory mediators, recruiting of systemic cells and vascular remodelling. To take advantage of this intricate response in the quest to devise new protective therapeutic strategies we need a better understanding of the territorial and temporal interplay between stroke-triggered inflammatory and cell death-inducing processes in both parenchymal and vascular brain cells. Our goal is to describe structural rearrangements and functional modifications occurring in glial and vascular cells early after an acute ischemic stroke. Low and high scale mapping of the glial activation on brain sections of mice subjected to 30 minutes middle cerebral artery occlusion (MCAO) was correlated with that of the neuronal cell death, with markers for microvascular changes and with markers for pro-inflammatory (IL-1β) and reparative (TGFβ1) cytokines. Our results illustrate a time-course of the neuroinflammatory response starting at early time-points (1 h) and up to one week after MCAO injury in mice, with an accurate spatial distribution of the observed phenomena.
Keywords
Animals, Brain/blood supply, Brain/metabolism, Brain/pathology, Brain/physiopathology, Cell Death, Disease Models, Animal, Humans, Inflammation/metabolism, Inflammation/pathology, Inflammation/physiopathology, Inflammation Mediators/metabolism, Interleukin-1beta/metabolism, Male, Mice, Neuroglia/metabolism, Neuroglia/pathology, Neurons/metabolism, Neurons/pathology, Stroke/metabolism, Stroke/pathology, Stroke/physiopathology, Transforming Growth Factor beta1/metabolism, Vascular Remodeling
Pubmed
Web of science
Open Access
Yes
Create date
07/02/2019 10:47
Last modification date
13/08/2020 6:21
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