Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe.

Details

Serval ID
serval:BIB_C3D2BB1EB767
Type
Article: article from journal or magazin.
Collection
Publications
Title
Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe.
Journal
Data in brief
Author(s)
Żurek-Biesiada D., Szczurek A.T., Prakash K., Best G., Mohana G.K., Lee H.K., Roignant J.Y., Dobrucki J.W., Cremer C., Birk U.
ISSN
2352-3409 (Print)
ISSN-L
2352-3409
Publication state
Published
Issued date
06/2016
Peer-reviewed
Oui
Volume
7
Pages
157-171
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Single Molecule Localization Microscopy (SMLM) is a recently emerged optical imaging method that was shown to achieve a resolution in the order of tens of nanometers in intact cells. Novel high resolution imaging methods might be crucial for understanding of how the chromatin, a complex of DNA and proteins, is arranged in the eukaryotic cell nucleus. Such an approach utilizing switching of a fluorescent, DNA-binding dye Vybrant® DyeCycle™ Violet has been previously demonstrated by us (Żurek-Biesiada et al., 2015) [1]. Here we provide quantitative information on the influence of the chemical environment on the behavior of the dye, discuss the variability in the DNA-associated signal density, and demonstrate direct proof of enhanced structural resolution. Furthermore, we compare different visualization approaches. Finally, we describe various opportunities of multicolor DNA/SMLM imaging in eukaryotic cell nuclei.
Keywords
Chromatin, DNA, DNA dye, Fluorescence, Localization microscopy, Nucleus, Single molecules, Super-Resolution, Vybrant violet, dSTORM
Pubmed
Web of science
Open Access
Yes
Create date
28/10/2019 12:55
Last modification date
29/10/2019 6:26
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