Routine next generation sequencing of lymphoid malignancies: clinical utility and challenges from a 3-Year practical experience.

Details

Serval ID
serval:BIB_C3B55B7BAB5C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Routine next generation sequencing of lymphoid malignancies: clinical utility and challenges from a 3-Year practical experience.
Journal
Leukemia & lymphoma
Author(s)
Pillonel V., Juskevicius D., Bihl M., Stenner F., Halter J.P., Dirnhofer S., Tzankov A.
ISSN
1029-2403 (Electronic)
ISSN-L
1026-8022
Publication state
Published
Issued date
11/2020
Peer-reviewed
Oui
Volume
61
Number
11
Pages
2568-2583
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Since 2016, a next-generation sequencing (NGS) panel targeting 68 genes frequently mutated in lymphoid malignancies is an accredited part of routine diagnostics at the Institute of Pathology in Basel, Switzerland. Here, we retrospectively evaluate the feasibility and utility of integrating this NGS platform into routine practice on 80 diagnostic cases of lymphoid proliferations. NGS analysis was useful in most instances, yielding a diagnostically, predictively and/or prognostically meaningful result. In 35 out of the 50 cases, in which conventional histopathological evaluation remained indecisive, molecular subtyping with the NGS panel was helpful to either confirm or support the favored diagnosis, enable a differential diagnosis, or seriously question a suspected diagnosis. A total of 61 actionable or potentially actionable mutations in 34 out of 80 cases that might have enabled patient selection for targeted therapies was detected. NGS panel analysis had implications for prognosis in all 15 cases interrogated for risk assessment.
Keywords
High-Throughput Nucleotide Sequencing, Humans, Mutation, Neoplasms, Prognosis, Retrospective Studies, Gene panel testing, diagnostic molecular pathology, lymphoma, next generation sequencing
Pubmed
Web of science
Create date
23/05/2024 10:59
Last modification date
24/05/2024 7:05
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