Dietary (n-3) polyunsaturated fatty acids affect the kinetics of pro- and antiinflammatory responses in mice with Pseudomonas aeruginosa lung infection

Details

Serval ID
serval:BIB_C3889370C212
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dietary (n-3) polyunsaturated fatty acids affect the kinetics of pro- and antiinflammatory responses in mice with Pseudomonas aeruginosa lung infection
Journal
J Nutr
Author(s)
Tiesset H., Pierre M., Desseyn J. L., Guery B., Beermann C., Galabert C., Gottrand F., Husson M. O.
ISSN
1541-6100 (Electronic)
ISSN-L
0022-3166
Publication state
Published
Issued date
01/2009
Volume
139
Number
1
Pages
82-9
Language
english
Notes
Tiesset, Helene
Pierre, Maud
Desseyn, Jean-Luc
Guery, Benoit
Beermann, Christopher
Galabert, Claude
Gottrand, Frederic
Husson, Marie-Odile
eng
Research Support, Non-U.S. Gov't
J Nutr. 2009 Jan;139(1):82-9. doi: 10.3945/jn.108.096115. Epub 2008 Dec 3.
Abstract
The underlying mechanisms by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) affect host resistance to Pseudomonas aeruginosa are unclear. The aim of this study was to determine their role on the kinetic of pro- and antiinflammatory response in lung infection. Mice fed either a control diet or a diet enriched with EPA and DHA were infected intratracheally and we studied lung expression of proinflammatory markers [CXCL1, interleukin (IL)-6, tumor necrosis factor-alpha], antiinflammatory markers (IL-10, A20, and IkappaB alpha), and PPARalpha and PPARgamma. The inflammatory response was assessed using recruitment of neutrophils and macrophages into bronchoalveolar lavage fluid, bacterial clearance from the lung, pulmonary injury, and 7-d survival rate. Compared with the control group, EPA and DHA delayed the expression of proinflammatory markers during the first 2 h (P < 0.05), upregulated proinflammatory marker expression (P < 0.05), and induced overexpression of antiinflammatory markers at 8 h (P < 0.05), enhanced recruitment of neutrophils at 16 h (P < 0.05), and induced PPARalpha and PPARgamma overexpression at 4 and 8 h (P < 0.01), respectively. Pulmonary bacterial load decreased and pulmonary injury and mortality were reduced during the first 24 h (P < 0.05). In conclusion, EPA and DHA modulate the balance between pro- and antiinflammatory cytokines, alter the early response of the host to P. aeruginosa infection, and affect the early outcome of infection.
Keywords
Animals, Bronchoalveolar Lavage Fluid/cytology, Diet, Fatty Acids, Omega-3/*pharmacology, Gene Expression Profiling, Gene Expression Regulation/drug effects, Macrophages/metabolism, Male, Mice, Mice, Inbred C57BL, Neurturin, Pneumonia, Bacterial/*metabolism/microbiology, Pseudomonas Infections/*metabolism/microbiology, *Pseudomonas aeruginosa, Time
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
Usage data