Expressed isolated integrin beta1 subunit cytodomain induces endothelial cell death secondary to detachment.

Details

Serval ID
serval:BIB_C36776C34A30
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expressed isolated integrin beta1 subunit cytodomain induces endothelial cell death secondary to detachment.
Journal
Thrombosis and Haemostasis
Author(s)
Hasmim M., Vassalli G., Alghisi G.C., Bamat J., Ponsonnet L., Bieler G., Bonnard C., Paroz C., Oguey D., Rüegg C.
ISSN
0340-6245
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
94
Number
5
Pages
1060-1070
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Expression of isolated beta integrin cytoplasmic domains in cultured endothelial cells was reported to induce cell detachment and death. To test whether cell death was the cause or the consequence of cell detachment, we expressed isolated integrin beta1 cytoplasmic and transmembrane domains (CH1) in cultured human umbilical vein endothelial cells (HUVEC), and monitored detachment, viability, caspase activation and signaling. CH1 expression induced dose-dependent cell detachment. At 24 h over 90% of CH1-expressing HUVEC were detached but largely viable (>85%). No evidence of pro-caspase-8,-3, and PARP cleavage or suppression of phosphorylation of ERK, PKB and Ikappa-B was observed. The caspase inhibitor z-VAD did not prevent cell detachment. At 48 h, however, CH1-expressing cells were over 50% dead. As a comparison trypsin-mediated detachment resulted in a time-dependent cell death, paralleled by caspase-3 activation and suppression of ERK, PKB and Ikappa-B phosphoyrylation at 24 h or later after detachment. HUVEC stimulation with agents that strengthen integrin-mediated adhesion (i.e. PMA, the Src inhibitor PP2 and COMP-Ang1) did not prevent CH1-induced detachment. Expression of CH1 in rat carotid artery endothelial cells in vivo caused endothelial cell detachment and increased nuclear DNA fragmentation among detached cells. A construct lacking the integrin cytoplasmic domain (CH2) had no effect on adhesion and cell viability in vitro and in vivo. These results demonstrate that isolated beta1 cytoplasmic domain expression induces caspase-independent detachment of viable endothelial cells and that death is secondary to detachment (i.e. anoikis). They also reveal an essential role for integrins in the adhesion and survival of quiescent endothelial cells in vivo.
Keywords
Animals, Anoikis, Antigens, CD29, Carotid Arteries, Caspase 3, Caspase 8, Caspases, Cell Adhesion, Cell Survival, Cells, Cultured, DNA Fragmentation, Endothelium, Vascular, Extracellular Signal-Regulated MAP Kinases, Gene Expression, Humans, I-kappa B Proteins, MAP Kinase Signaling System, Protein Structure, Tertiary, Protein Subunits, Proto-Oncogene Proteins c-akt, Rats, Umbilical Veins
Pubmed
Web of science
Create date
28/01/2008 8:36
Last modification date
20/08/2019 15:38
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